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MTK234_015

Plasmid

#123991

Purpose

Encodes the spCas9 sgRNA1 hROSA26 (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hROSA26 sg4

Expression

Mammalian

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only

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MTK8_005

Plasmid

#123885

Purpose

Encodes the F1 Kanamycin resistance cassette as a type 8 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

F1-KanR-ColE1

Expression

Mammalian

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_021

Plasmid

#123936

Purpose

Encodes the spacer TU with connectors L3 R4 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL3R4 spacer

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_047

Plasmid

#124055

Purpose

Encodes the spCas9 mScarlet (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_048

Plasmid

#124056

Purpose

Encodes the spCas9 mScarlet (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_049

Plasmid

#124057

Purpose

Encodes the spCas9 mScarlet (site 4) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

mScarlet sgRNA 4

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ARB340

Plasmid

#124023

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::iRFP713-bgPA_R1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB341

Plasmid

#124024

Purpose

Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LS-A4_EF1a-NLS::tagBFP-bgPA_R1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB344

Plasmid

#124027

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::iRFP713-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB345

Plasmid

#124028

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic tagBFP

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::tagBFP-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB346

Plasmid

#124029

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mAzamiGreen

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::mAzamiGreen-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB347

Plasmid

#124030

Purpose

Encodes TU with connectors L1 R2 and an B1 insulator, and pCMV expressing cytoplasmic mRuby2

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L1-B1_CMV-NES::mRuby2-rglPA_R2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB348

Plasmid

#124031

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged iRFP713

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::iRFP713-SV40_RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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ARB350

Plasmid

#124033

Purpose

Encodes TU with connectors L2 R3 and an C3 insulator, and pCAG expressing membrane-tagged mAzamiGreen

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

L2-C3_CAG-Lyn::mAzamiGreen-SV40_RE

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0455

Plasmid

#124040

Purpose

Encodes pPGK1 driving expression of cytoplasmic tagBFP in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-NES-tagBFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0456

Plasmid

#124041

Purpose

Encodes pPGK1 driving expression of mScarlet in a 2nd gen. Lenti Virus destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pHR_pPGK1-mScarlet

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_023

Plasmid

#123953

Purpose

Encodes human RelA as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RelA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_025

Plasmid

#123954

Purpose

Encodes synTF fused to VP16 activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SynTF_VP16

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK3b_026

Plasmid

#123955

Purpose

Encodes synTF fused to VP64 activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SynTF_VP64

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK4a_009

Plasmid

#123956

Purpose

Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

T2A::NLS::EBFP2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_009

Plasmid

#123957

Purpose

Encodes the spCas9 pC120 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

C120 guide

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_011

Plasmid

#123958

Purpose

Encodes the spCas9 pPLMP1 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

plmp guide A1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_054

Plasmid

#123959

Purpose

Encodes the saCas9 CXCR1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

CXCR4 sa sgRNA 3

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_009

Plasmid

#123964

Purpose

Encodes PhiC31 landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phiC31 landing

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_010

Plasmid

#123965

Purpose

Encodes BxBI landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI landing

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_033

Plasmid

#123971

Purpose

Encodes the cROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

cROSA26 PhiC31 LP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_034

Plasmid

#123972

Purpose

Encodes the hROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hROSA26 PhiC31 LP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_036

Plasmid

#123973

Purpose

Encodes the cROSA26 BxBI landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

cROSA26 BxBI LP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK0_045

Plasmid

#123975

Purpose

Encodes the cROSA26 destination with hygyomycin resistance cassette as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

cROSA26 DEST (hygro)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MTK234_057

Plasmid

#123982

Purpose

Encodes the spCas9 Safetargeting Chr20 gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sg Safetargeting Chr20

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge