We narrowed to 14,200 results for: cas9

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pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Publication

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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SauCas9 EMX1-nicking sgRNA

Plasmid

#169860

Purpose

SauCas9 nicking sgRNA for EMX1

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9 EMX1-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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SauCas9KKH CCR5-nicking sgRNA

Plasmid

#169863

Purpose

SauCas9KKH nicking sgRNA for CCR5

Depositor

Scot Wolfe

Publication

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SauCas9KKH CCR5-nicking sgRNA

Expression

Mammalian

Promoter

human U6 promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNArodA

Plasmid

#149656

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pD2-dcas9-J23101-GFP

Plasmid

#122260

Purpose

Constitutive dCas9 and GFP expression plasmid

Depositor

Chueh Loo Poh

Publication

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

dCas9

Use

Synthetic Biology

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-FLEX-SaCas9-U6-sgGabrg3

Plasmid

#124858

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2a

Plasmid

#124863

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

Plasmid

#124859

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGria3

Plasmid

#124869

Purpose

Mutagenesis of Gria3

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gria3 (Gria3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg3

Plasmid

#124872

Purpose

Mutagenesis of Gabrg3

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg3 (Gabrg3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGabrg2

Plasmid

#124871

Purpose

Mutagenesis of Gabrg2

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Gabrg2 (Gabrg2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Publication

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

Plasmid

#202555

Purpose

Synaptotagmin-1 sgRNA2 with TagBFP2

Depositor

Frederic Meunier

Publication

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

Plasmid

#202556

Purpose

Synaptotagmin-1 sgRNA3 with TagBFP2

Depositor

Frederic Meunier

Publication

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

Plasmid

#202554

Purpose

Synaptotagmin-1 sgRNA1 with TagBFP2

Depositor

Frederic Meunier

Publication

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

Plasmid

#68347

Purpose

AAV plasmid expressing the FlpO recombinase. with empty gRNA

Depositor

Jannik Elverløv-Jakobsen

Insert

FlPO

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Publication

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Promoter

anhydrotetracycline Ptet promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV-SaPE2

Plasmid

#174817

Purpose

Mammalian expression of SaCas9 prime editor 2

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

SaPE2

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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