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MXS_MCS4

Plasmid

#62398

Purpose

MXS_chaining vector with MCS4

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

MluI-XhoI-PvuII-BglII-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGLx2-set1-H1017K

Plasmid

#53258

Purpose

GAL driven LexA-tagged SET1-catalytically dead (H1017K)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Tags

LexA

Expression

Yeast

Mutation

H1017K mutation - catalytically dead

Promoter

GAL1/10

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBACgus N-His/GST/LRRK2 1867-2141

Plasmid

#40404

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2141

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus N-His/GST/LRRK2 1867-2161

Plasmid

#40405

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

GST-Tev and HisTev

Mutation

WT aa1867-2161

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S

Plasmid

#40372

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

HisTev

Mutation

aa1867-2161, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S

Plasmid

#40388

Depositor

Michael J Fox Foundation MJFF

Inserts

SUMO
LRRK2 (LRRK2 Human)

Use

Baculovirus

Tags

6xHis and HisTEV

Mutation

aa1867-2186, C2024S/C2025S

Promoter

polH

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pTH490-tQSRTL

Plasmid

#29699

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

Insert

tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G

Expression

Yeast

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pWZ414-F48

Plasmid

#23072

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Glutamine, H3 K14…

Available Since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F50

Plasmid

#23074

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 14 to Arginine, H3 K14R…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F54

Plasmid

#23078

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO SSA1

Plasmid

#19462

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A

Plasmid

#18691

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

Plasmid

#14849

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

VYFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

Plasmid

#14850

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)

Tags

ECFP

Expression

Yeast

Mutation

TATA-A1T6. TATA box mutated from TATATAAG to AATA…

Available Since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
Ypd1p-pET22b

Plasmid

#12655

Depositor

SeWon Suh

Article

Song et al J Mol Biol. 1999 Nov 5. 293(4):753-61.

Insert

YPD1 protein (YPD1 Budding Yeast)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
WT Ecm2

Plasmid

#169922

Purpose

Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

Ecm2

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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mPiezo1-IRES-eGFP

Plasmid

#80925

Purpose

expresses mouse Piezo1 with GFP

Depositor

Ardem Patapoutian

Article

Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

Insert

mouse Piezo1 CDS (Piezo1 Mouse)

Expression

Mammalian

Available Since

Nov. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NtC60αC20NtLC60β

Plasmid

#160877

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Expression

Bacterial

Promoter

rbcL: T7, chaperones: T7

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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