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His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Publication

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroME(F243Y)-mRuby2-ST

Plasmid

#109127

Purpose

Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Publication

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME(F243Y)-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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P16ΔS:S10-(MCS)

Plasmid

#108182

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Publication

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Bottom-T

Plasmid

#87313

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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B0032m_BC

Plasmid

#66020

Purpose

MoClo Basic Part: RBS - Weiss RBS, medium strength. Modified from Bba_B0032 to adjust spacing in MC system. [B:B0032m:C]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

RBS

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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cre_CD

Plasmid

#66030

Purpose

MoClo Basic Part: CDS - cre recombinase (recombines DNA at loxP sites) [C:cre:D]

Depositor

Douglas Densmore

Publication

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Recombinase

Use

Synthetic Biology

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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LI A-B EF1 c2

Plasmid

#54435

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

promoter region of A. thaliana EF1alpha1 /AT1G07940.1

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pJAF214

Plasmid

#52404

Purpose

Expresses Flag-tagged mouse Arf3

Depositor

Gregory Pazour

Publication

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf3 (Arf3 Mouse)

Tags

Flag

Expression

Mammalian

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
pSuper 44-1

Plasmid

#51597

Purpose

shRNA 1 against rat and mouse Sema4D

Depositor

Suzanne Paradis

Publication

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA1

Use

RNAi

Promoter

U6

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
TAL2040

Plasmid

#39398

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-21-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL2004

Plasmid

#39362

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-03-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2005

Plasmid

#39363

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-03-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2051

Plasmid

#39409

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-26-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TAL2006

Plasmid

#39364

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-04-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2093

Plasmid

#39427

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-35-Right

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2092

Plasmid

#39426

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-35-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2060

Plasmid

#39418

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-31-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2008

Plasmid

#39366

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-05-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only