We narrowed to 32,111 results for: eng

1. pDYH5

   Plasmid

   #52032

   Purpose

   Expression of GFP-tagged DAF-16 in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

   Insert

   DAF-16 (daf-16 Nematode)
   

   Tags

   GFP and HIS

   Expression

   Worm

   Available Since

   May 6, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pYPQAT43

   Plasmid

   #223415

   Purpose

   T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for monocot plants; TTTV PAM; LbCas12a-D156R and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-LbCas12a-D156R-ZmUbi-crRNA scaffold
   

   Use

   CRISPR; T-dna vector

   Tags

   NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. single cut NH-donor

   Plasmid

   #83575

   Purpose

   eGFP reporter donor containing single SA cutting site for NHEJ knock-in

   Depositor

   Bo Feng

   Article

   He et al Nucleic Acids Res. 2016 May 19;44(9):e85

   Insert

   eGFP
   

   Use

   Nhej donor

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-PIS

   Plasmid

   #119078

   Purpose

   Visualize localization of ER enzyme Phosphatidylinositol Synthase (PIS)

   Depositor

   Gia Voeltz

   Article

   English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

   Insert

   PIS (CDIPT Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tol2-krt4-GFP

   Plasmid

   #97151

   Purpose

   label apical epithelial cells in zebrafish

   Depositor

   Qing Deng

   Article

   Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

   Insert

   AcGFP
   

   Use

   Zebrafish

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT56

   Plasmid

   #223428

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLS.492

   Plasmid

   #184957

   Purpose

   Test effect of a1/a2 length on editing rpoB using retron recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
   

   Expression

   Bacterial

   Mutation

   rpoB donor T1534C, a1/a2 length extended to 22 bp

   Promoter

   T7/lac

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTK_Cerulean_Nanotag_70_neg_Control

   Plasmid

   #130568

   Purpose

   Enhancer cloning nanotag reporter vector

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

   Insert

   negative oligo sequence
   

   Use

   Enhancer cloning nanotag reporter vector

   Promoter

   thymidine kinase minimal promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQAT39

    Plasmid

    #223411

    Purpose

    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants select.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA 2 GAPDH

    Plasmid

    #83809

    Purpose

    Express Sg-2 sgRNA targeting GAPDH

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    SgRNA
    

    Use

    CRISPR

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP_sfCherry2(11)_HP1

    Plasmid

    #82607

    Purpose

    Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_HP1b
    

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pTrex-n-eGFP

    Plasmid

    #62544

    Purpose

    Expression of eGFP in T. cruzi. The NheI-linearized form of this plasmid could be efficiently integrated in to T. cruzi genome

    Depositor

    Rick Tarleton

    Article

    Peng et al MBio. 2014 Dec 30;6(1). pii: e02097-14.

    Insert

    eGFP
    

    Use

    (1)trypanosoma cruzi expression of egfp (2) genom…

    Tags

    None

    Mutation

    None

    Promoter

    Ribo-HX1

    Available Since

    April 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pFLAG-XBP1u-FLuc

    Plasmid

    #31239

    Depositor

    Jimtong Horng

    Article

    Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

    Insert

    FLAG-XBP1u-FLuc (XBP1 Human)
    

    Tags

    Flag and Fluc

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pEB-p53shRNA

    Plasmid

    #28222

    Depositor

    Linzhao Cheng

    Article

    Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

    Insert

    p53 shRNA
    

    Expression

    Mammalian

    Available Since

    March 25, 2011

    Availability

    Academic Institutions and Nonprofits only

16. EF.hICN1.Ubc.GFP

    Plasmid

    #17626

    Depositor

    Linzhao Cheng

    Article

    Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

    Insert

    ICN1 (NOTCH1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQAT45

    Plasmid

    #223417

    Purpose

    T-DNA vector for temperature tolerance LbCas12a-D156R based mutagenesis for dicot plants; TTTV PAM; LbCas12a-D156R and the crRNA was driven by separate 2x35s promoter; Kanamycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    2x35s-LbCas12a-D156R-2x35s-crRNA scaffold
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEZ-1

    Plasmid

    #51868

    Purpose

    Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

    Insert

    psdhb-1::mtLS-cpYFP
    

    Expression

    Worm

    Promoter

    sdhb-1

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQAT59

    Plasmid

    #223431

    Purpose

    T-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB1_hPMS2

    Plasmid

    #129425

    Purpose

    Expression human PMS2 protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human PMS2 (PMS2 Human)
    

    Expression

    Insect

    Promoter

    Polyhedrin promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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