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pSG 163

Plasmid

#63871

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT2

Plasmid

#67476

Purpose

Expression of human N-myristoyltransferase 2 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT2 (NMT2 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCD175

Plasmid

#113316

Purpose

dCas9, MCP-AsiA, rpoD_F563Y

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-T4.AsiA, rpoD_F563Y

Use

CRISPR

Expression

Bacterial

Mutation

F563Y

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCD296.7

Plasmid

#113320

Purpose

dCas9, MCP-TetD, J107 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, MCP-TetD, J107 scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-ftyrp>TRAP

Plasmid

#42299

Depositor

Stephen L Johnson

Article

Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

Insert

EGFP-Rpl10a (rpl10a Zebrafish)

Tags

EGFP

Promoter

Takifugu rubripes tyrosine related protein 1 (tyr…

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSTC0

Plasmid

#39240

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSG lac10

Plasmid

#63875

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pJF121

Plasmid

#113325

Purpose

pTet-W108 scRNA.b2

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

W108 scRNA_MS2

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pSTC1

Plasmid

#39241

Depositor

Alfonso Jaramillo

Article

Rodrigo et al Proc Natl Acad Sci U S A. 2012 Sep 18;10

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

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lvl0-I-E1

Plasmid

#229161

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

E1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-F1

Plasmid

#229162

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

F1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I [backbone]

Plasmid

#229153

Purpose

Insulator backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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DOX: FSHB-t2a-GFP

Plasmid

#205595

Purpose

an overexpression vector of the killifish FSHB under doxycycline control

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

FSHB

Tags

GFP

Promoter

Ef1a

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJZC530

Plasmid

#135434

Purpose

Yeast integrating plasmid for pTETO1-Venus reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

pTETO1_Venus

Expression

Yeast

Promoter

pTETO1

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2

Plasmid

#149428

Purpose

cloning vector with regulatory regions of the murine Tdg gene

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox

Expression

Mammalian

Promoter

murine Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJS032

Plasmid

#135431

Purpose

Yeast integrating plasmid to express PYL1-VP64

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

PYL1-VP64

Expression

Yeast

Promoter

pAdh

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJS033

Plasmid

#135432

Purpose

Yeast integrating plasmid to express MCP-ABI

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

MCP-ABI

Expression

Yeast

Promoter

pTEF1

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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NK651

Plasmid

#109107

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

PTSG_07215 deltaCC

Use

Choanoflagellate expression

Mutation

Deletes C-terminal coiled-coil from PTSG_07215

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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