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pKM493

Plasmid

#140191

Purpose

ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

TEV-Flag-Gly4-eGFP

Tags

Flag + eGFP

Expression

Bacterial

Promoter

PGroEL

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi2-6

Plasmid

#197706

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi2 promoter

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmAXIG1-6

Plasmid

#197711

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmAXIG1 promoter

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmPLTP-6

Plasmid

#197713

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmPLTP promoter

Expression

Plant

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcS-p88-C1C2

Plasmid

#178424

Purpose

p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

Depositor

Masako Harada

Publication

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p88

Expression

Mammalian

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-gLuc-C1C2

Plasmid

#178426

Purpose

Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Publication

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gLuc

Expression

Mammalian

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pPI459

Plasmid

#113196

Purpose

extrachromosomal expression vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector, extra…

Tags

mScarlet

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI437

Plasmid

#113195

Purpose

extrachromosomal expression vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector, extra…

Tags

mScarlet

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1513

Plasmid

#108998

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

GFP

Available since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM1515

Plasmid

#109000

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

GFP

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDM1514

Plasmid

#108999

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

mCherry

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTCW007

Plasmid

#83525

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 1 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Publication

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha1

Expression

Yeast

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929582

Plasmid

#38169

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pGGC012

Plasmid

#48826

Purpose

Provides GFP:NLS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP:NLS

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAWP78-PVCpnf_pvc13-Ad5_HUHe-Pvc10-Cas9

Plasmid

#243744

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on NTD and Cas9 on CTD

Depositor

Feng Zhang

Publication

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_HUHe-Pvc10-Cas9

Expression

Bacterial

Available since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

haA3A-CBE-G

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Neq2X

Plasmid

#182365

Purpose

Expresses Neq2X DNA polymerase

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK1009 (pSL9)

Plasmid

#191004

Purpose

Expresses E2 Crimson on a broad host range pBBR1 origin plasmid

Depositor

Jeffrey Barrick

Publication

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA KanR neo

Plasmid

#167909

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Publication

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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