Addgene

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Showing: 4921 - 4940 of 9104 results

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pENTR-SYNZIP8

Plasmid

#80664

Purpose

Encodes the synthetic coiled coil peptide SYNZIP8

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP8

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP22

Plasmid

#80676

Purpose

Encodes the synthetic coiled coil peptide SYNZIP22

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP22

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pBL6

Plasmid

#50549

Purpose

Expresses mCherry under the PLtetO-1 promoter

Depositor

Jeffrey Tabor

Article

Olson et al Nat Methods. 2014 Mar 9. doi: 10.1038/nm

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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p5E_(-3kb)nrg1 type II

Plasmid

#199393

Purpose

pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

(-3kb)nrg1 type II

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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p5E_ngn1_LSE_C

Plasmid

#199395

Purpose

pDEL98; Gateway 5' Entry clone encoding select ngn1 upstream regulatory sequence

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

LSE_C

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ134B

Plasmid

#179216

Purpose

Golden Gate entry vector; 4th gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU3

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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DUX4 pDonor221

Plasmid

#187801

Purpose

DUX4 entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGIG

Plasmid

#11159

Purpose

Mammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

IRES-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-slc1a3b

Plasmid

#170205

Purpose

Gateway 5’ entry vector with the slc1a3b promoter

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b )

Use

Unspecified; Zebrafish

Tags

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi:loxP-EGFP-loxP

Plasmid

#27322

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

ubiquitin:loxP-EGFP-loxP

Use

Multisite gateway 5' entry vector

Tags

Expression

Mutation

Promoter

Available since

April 5, 2011

Availability

Academic Institutions and Nonprofits only

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pV1090

Plasmid

#111427

Purpose

gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

sgRNA-BsmBI stuffer

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK009-3a-αAmylase-αMFΔ

Plasmid

#84968

Purpose

Type 3a, α-Amylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-Amylase followed by MFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pPTK010-3a-Glucoamylase-αMFΔ

Plasmid

#84969

Purpose

Type 3a, Glucoamylase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glucoamylase followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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C122-E11: eGFP

Plasmid

#162893

Purpose

Standard Gateway entry clone encoding eGFP fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

enhanced Green fluorescent protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA

Plasmid

#179211

Purpose

Golden Gate entry vector; 2nd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

no promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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JD630

Plasmid

#160394

Purpose

Vitis GRF4-GIF1 in pDONR-zeo

Depositor

Jorge Dubcovsky

Article

Debernardi et al Nat Biotechnol. 2020 Oct 12. pii: 10.103

Insert

Vitis GRF4-GIF1 chimera

Use

Entry clon

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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dg263_pENTR_ChrimsonCel

Plasmid

#66101

Purpose

Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chrimson

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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