We narrowed to 16,165 results for: igh

1. pLIB-RSP23

   Plasmid

   #176702

   Purpose

   Expression of Chlamydomonas reinhardtii RSP23 recombinant protein in insect cells

   Depositor

   Ron Vale

   Article

   Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

   Insert

   RSP23
   

   Use

   Tags

   Expression

   Insect

   Mutation

   None

   Promoter

   polyhedrin

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dynein-2_complex

   Plasmid

   #132536

   Purpose

   Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Inserts

   DYNC2H1 (DYNC2H1 Human)
   DYNC2LI1 (DYNC2LI1 Human)
   WDR34 (WDR34 Human)
   WDR60 (WDR60 Human)
   DYNLL1 (DYNLL1 Human)
   DYNLL2 (DYNLL2 Human)
   DYNLRB1 (DYNLRB1 )
   DYNLRB2 (DYNLRB2 Human)
   DYNLT1 (DYNLT1 Human)
   TCTEX1D2 (TCTEX1D2 Human)
   DYNLT3 (DYNLT3 Human)
   LC8-like (LOC392067 Human)
   

   Use

   Tags

   8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #209725

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD_TypeIC_Cascade_cas3_invivo

   Plasmid

   #196400

   Purpose

   expresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assay

   Depositor

   David Taylor

   Article

   O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

   Inserts

   Cas5c
   Cas7c
   Cas8c
   Cas3c
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCMV Puro DEST ERKKTRClover

   Plasmid

   #59150

   Purpose

   Lentiviral vector to express ERK KTR mClover under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   ERK Kinase Translocation Reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Bxb1-GT_CAG-mRuby2

   Plasmid

   #229801

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mRuby2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIVT-eeBxb1

   Plasmid

   #232748

   Purpose

   IVT template for making modRNA encoding eeBxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   V74A, E229K, V375I

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIVT-Flp-2A-BleoR

    Plasmid

    #232752

    Purpose

    IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Inserts

    FlpO
    BleoR
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVT-iCre

    Plasmid

    #232773

    Purpose

    IVT template for making modRNA encoding iCre

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    iCre
    

    Use

    Cre/Lox; In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hsyn-GRAB_HA1h

    Plasmid

    #190473

    Purpose

    Express the high-affinity histamine sensor GRAB_HA1h in neurons

    Depositor

    Yulong Li

    Article

    Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

    Insert

    Histamine sensor GRAB_HA1h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMART-HCKan-yibDp-VHH-VCAM1

    Plasmid

    #202471

    Purpose

    Expresses the nanobody VCAM1 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VCAM1
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTC217

    Plasmid

    #70018

    Purpose

    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b

    Depositor

    Daniel Voytas

    Article

    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

    Insert

    Nuclease (Cas9/sgRNA) + Donor + GVR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-PuroR

    Plasmid

    #232753

    Purpose

    IVT template for making modRNA encoding the Puromycin resistance gene

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    PuroR
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1-GA_CAG-mStayGold

    Plasmid

    #229802

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRPF215

    Plasmid

    #106377

    Purpose

    C. difficile transposon mutagenesis system. Shuttle vector containing tet-inducible Himar1 transposase and custom ErmR transposon

    Depositor

    Neil Fairweather, Robert Fagan

    Article

    Dembek et al MBio. 2015 Feb 24;6(2):e02383. doi: 10.1

    Insert

    Ptet-himar1-Ter(slpA)-transposon
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Codon optimised for C. difficile

    Promoter

    Ptet

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti TurboRFP BlastR

    Plasmid

    #102343

    Purpose

    Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Tags

    N/A

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Bxb1-GA_CAG-EGFP

    Plasmid

    #229799

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

    Plasmid

    #209726

    Purpose

    Expresses cytosolic medium affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-Cre

    Plasmid

    #123133

    Purpose

    Mammalian expression vector for wild-type Cre.

    Depositor

    David Liu

    Article

    Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SPDK3959

    Plasmid

    #199246

    Purpose

    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

    Insert

    Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence
    

    Use

    T-dna vector

    Tags

    Expression

    Mutation

    Promoter

    PEBV

    Available since

    June 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lenti-PEAR-mCherry

    Plasmid

    #228892

    Purpose

    All-in-one lentiviral version of the PEAR reporter. Constitutive RFP expression with GFP turned on in event of prime editing.

    Depositor

    Francisco J Sánchez-Rivera

    Article

    Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

    Insert

    EGFP-IRES-mScarlet
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRSET FlipSuc-90u delta1

    Plasmid

    #14475

    Depositor

    Wolf Frommer

    Article

    Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

    Insert

    FlipThuE W283A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    N192D, W283A, 13 amino acids deleted from linkers

    Promoter

    T7

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SpCas9-CBE6b

    Plasmid

    #215820

    Purpose

    Express CBE6b (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI (cas9 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge