...LEU13 IGH@ immunoglobulin heavy locus DKFZp686C15213, IGH, IGH.1@, IGHDY1, MGC72071, MGC88774 IGHA1 immunoglobulin...diversity 7-27 DHQ52, IGHD727 IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy...variable 1-58 IGHV158, VH IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin...-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin...-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304 IGHV4-31 immunoglobulin...diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-functional) DM2, IGHD114 IGHD1-20 immunoglobulin...heavy diversity 1-20 IGHD120 IGHD1-26 immunoglobulin heavy diversity 1-26 IGHD126 IGHD1-7 immunoglobulin ...

...-TALEN-01-Right TAL2001 TGAACAGCTC eGFP-TALEN-02-Left TAL2002 TGGTGCCCAT eGFP-TALEN-02-Right TAL2003 TTACGTCGCC...-TALEN-03-Right TAL2005 TGGGCCAGGG eGFP-TALEN-04-Left TAL2006 TGGAGTACAA eGFP-TALEN-04-Right TAL2007 TGATATAGAC...eGFP-TALEN-05-Right TAL2009 TAGGTGGCATC eGFP-TALEN-06-Left TAL2010 TGCCACCTACG eGFP-TALEN-06-Right TAL2011 ...eGFP-TALEN-07-Right TAL2013 TGCACGCCGTA eGFP-TALEN-08-Left TAL2014 TTCAAGATCCG eGFP-TALEN-08-Right TAL2015 ...eGFP-TALEN-09-Right TAL2017 TCGCCCTTGCTC eGFP-TALEN-10-Left TAL2018 TCCGGCGAGGGC eGFP-TALEN-10-Right TAL2019...eGFP-TALEN-11-Right TAL2021 TGGTCGGGGTAG eGFP-TALEN-12-Left TAL2022 TCCGCCACAACA eGFP-TALEN-12-Right TAL2023...eGFP-TALEN-13-Right TAL2025 TGAACTTGTGGCC eGFP-TALEN-14-Left TAL2026 TCAGCCGCTACCC eGFP-TALEN-14-Right TAL2027...

... our collection! AAV are distributed as high-quality and high-titer 100 µL solutions of purified viral...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper...AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin...AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...

...Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance...transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017 Jun 29...Red genetically encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors ...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...organelle-localized). Browse or search the tables below highlighting a few popular categories of biosensors or browse...NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924...for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: ...

...constitutively express your gene of interest at high levels. Perhaps high expression leads to slow bacterial growth...pDawn 43796 FixK2 Blue Light (470 nm) Andreas Moeglich Empty vector for blue light inducible expression ... 43795 pR_FixK2 Blue Light (470 nm) Andreas Moeglich Empty vector for blue light repressible expression...protein of interest. pJT106b3 31393 Red light inducible inverter Red light (650 nm) Christopher Voigt When combined...PcpcG2 Green Light (532 nm) Christopher Voigt When combined with pPLPCB(S), encodes a green light responsive...for the production of large amounts of protein or high copy number plasmids. Whether you're studying the...individual protein’s function under a wide range of highly controlled conditions in vitro , or, if you want...

...pAAV-hSyn-dLight1.1 Syn dLight1.1 none Constitutive 1 Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive...pAAV-hSyn-dLight1.2 Syn dLight1.2 none Constitutive 1, 5 Tian 125560 AAV-CAG-dLight1.3b CAG dLight1.3b none...pAAV-syn-dLight1.3b Syn dLight1.3b none Constitutive 9 Tian 125707 pAAV-CAG-RdLight1 CAG RdLight1 none Constitutive...iAChSnFR Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors...Constitutive 9 Tian 125708 pAAV-hSynapsin1-RdLight1 Syn RdLight1 none Constitutive 9 Tian 113049 pAAV-hSyn-GRAB_DA1m...none Constitutive 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...

...enough to cause a DSB. If high specificity is crucial to your experiment, you might consider using the dual...will have the highest specificity. To increase specificity, you can also consider using a high-fidelity Cas...Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 8(11):2281-308. PMID: 24157548 High-frequency...end joining (NHEJ) pathway The less efficient but high-fidelity homology directed repair (HDR) pathway ...Break (Cut) Enhancing Specificity with Nickases and High Fidelity Enzymes CRISPR specificity is partially...researchers used rational mutagenesis to develop two high fidelity Cas9's : eSpCas9(1.1) and SpCas9-HF1. eSpCas9...interactions with the DNA phosphate backbone. Another high fidelity Cas9, HypaCas9 , was developed in 2017 ...

...the Fab fragments contain highly variable regions, shown in light blue and light orange, that bind to a ...are light-sensitive molecules which emit a specific wavelength of light after excitation by light of a...pair the brightness of the fluorophore with the abundance of the antibody target. If a bright fluorophore...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...-efficient way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies...protein chains: two large heavy chains and two small light chains. These four domains come together to form... to be produced in a single individual, giving a high probability that an antibody will be able to recognize...

...Excitation and Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and...low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that...Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 ...Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine ...Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids iLOV 447 497... Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527... Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange...

...Plasmids and Resources Plasmid Tools Collection Highlights Other Resources The zebrafish ( Danio rerio )...large populations that are amenable to genetics, high-throughput screening and non-invasive live imaging..., and drug discovery and development. Here we highlight some of the plasmids and tools available at Addgene... zebrafish gene or sequence . The table below highlights plasmids that contain tools for use in zebrafish...Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection CRISPR Please...control of gene expression in zebrafish using red light. LipoGlo - Steven Farber Lab. A reporter system ...lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab....

...applications. Browse the Collection Highlights section to learn more. Highlights of Luciferase Tools in our Collection...derived from teLuc that emit yellow/green light and red light, respectively, for increased usefulness in...Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression Constructs Reporter...application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more susceptible...their large size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available...red-shifted substrate Akalumine with significantly brighter signal and emission in the near-infrared range...pRSETb-pHlash : A pH sensor created by fusing a bright mutant of Renilla luciferase (Rluc8) through an...

...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent...pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive ...Deisseroth 137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...

... linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 5 amino acid linker V17V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 17 amino acid linker V32V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting of three Venus fluorescent...amino acid linker, respectively VVVV Anisotropy/brightness standard consisting of four Venus fluorescent...amino acid linker, respectively VVVVV Anisotropy/brightness standard consisting of five Venus fluorescent...connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard consisting of six Venus fluorescent ...

...) is expressed at higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven...plasmids. Addgene's viral service provides high-quality and high-titer viral preparations of plasmids that...Cre-containing Plasmids Scientists have developed ways to tightly control Cre expression and to optimize Cre expression...to activate Cre. One advantage of this system is tight temporal regulation. Promoter-regulated Cre: The...pmCherry-CRY2-CreN CRY2-CreN and mCherry coexpression; Light inducible; Use with plasmid 26889. Mammalian Tucker...pmCherry-CIBN-CreC CIBN-CreC and mCherry coexpression; Light inducible; Use with plasmid 26888. Mammalian Tucker...62938 pET-(pos36)GFP-9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre...

...(EXPRESs) Consortium You may also like... Gavin Wright Lab plasmids EXPRESs is a collection of mammalian...Institute Mitch Levesque University of Zürich Gavin Wright , Wellcome Sanger Institute Protocols: Video showing...extracellular receptor-ligand interactions. Kerr SJ and Wright GJ. J. Vis. Exp. (61), e3881, DOI: 10.3791/3881...O, Kwiatkowski DP, Duraisingh MT, Rayner JC and Wright GJ Nature 2011 Nov 9; 480(7378):534-7. Construction..., Adryan B, Thisse B, Thisse C, Teichmann S and Wright GJ Molecular & cellular proteomics 2010 Dec; 9(...neural leucine-rich repeat receptors. Söllner C and Wright GJ. Genome biology 2009; 10(9):R99. Large-scale..., Söllner C, Schuster-Boeckler B, Bateman A and Wright GJ. Genome research 2008 Apr; 18(4):622-30. A benchmarked...

.... In addition to being useful for our bodies to fight off intruders, antibodies are also extremely useful...to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use it to...and Expressing Antibodies The following table highlights plasmid collections that can be used to create...Article PI One-step assembly of antibody heavy- and light-chain DNAs into a single mammalian expression vector...Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv...Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...

...collections for use in plants. The tables below highlight plasmids spanning a wide variety of molecular ...The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, ... Li. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and...Return to top Empty Backbones The table below highlights plasmids that can be used as empty backbones ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent...

...iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging...near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background...iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective imaging of ...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

...proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific neurons...membrane channel that opens in response to blue light and allows cations to flow into the cell. Variants...cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as...or search the table below for plasmids that use light to control a protein or cellular activity. For example...

...Almost as small as NM, ST1 consistently yields slightly higher activities than the others as a nuclease and...and repression in bacteria, with NM exhibiting slightly superior repression. Treponema denticola (TD):... coli . Bacteria expressing NM and TD may grow slightly more slowly than those expressing SP and ST1. ...We experimentally determined these PAMs using a highly stringent selection, which revealed that PAM recognition...previously thought. In particular, combinations of highly unfavorable bases at positions adjacent to those...protospacers and applications requiring particularly tight binding. In these cases it may be advisable to use...