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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 88 results for: igh

Showing: 1 - 20 of 88 results

  1. Immunology Research Plasmids and Resources

    Type
    Collection
    ...LEU13 IGH@ immunoglobulin heavy locus DKFZp686C15213, IGH, IGH.1@, IGHDY1, MGC72071, MGC88774 IGHA1 immunoglobulin...diversity 7-27 DHQ52, IGHD727 IGHE immunoglobulin heavy constant epsilon IgE IGHG1 immunoglobulin heavy...variable 1-58 IGHV158, VH IGHV1-69 immunoglobulin heavy variable 1-69 IGHV1-E, IGHV169, IGHV1E IGHV1-8 immunoglobulin...-30-3 IGHV3-3, IGHV3303 IGHV3-30-5 immunoglobulin heavy variable 3-30-5 IGHV3-3, IGHV3305 IGHV3-33 immunoglobulin...-30-2 IGHV4-3, IGHV4302 IGHV4-30-4 immunoglobulin heavy variable 4-30-4 IGHV4-3, IGHV4304 IGHV4-31 immunoglobulin...diversity 1-1 IGHD11 IGHD1-14 immunoglobulin heavy diversity 1-14 (non-functional) DM2, IGHD114 IGHD1-20 immunoglobulin...heavy diversity 1-20 IGHD120 IGHD1-26 immunoglobulin heavy diversity 1-26 IGHD126 IGHD1-7 immunoglobulin ...

  2. Brain Initiative Collection

    Type
    Collection
    ... our collection! AAV are distributed as high-quality and high-titer 100 µL solutions of purified viral...coinjected with pAAV-TREtight-mTagBFP2-B19G Ian Wickersham 100799-AAV1 pAAV-TREtight-mTagBFP2-B19G helper...AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under synapsin...AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV1 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...AAV5 pAAV-hSyn-dLight1.2 To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance...transfer from chloroplasts to nuclei provides a high-light signalling mechanism. Nat Commun. 2017 Jun 29...Red genetically encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors ...to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage sensor to detect...organelle-localized). Browse or search the tables below highlighting a few popular categories of biosensors or browse...NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924...for Ex Vivo and In Vivo Imaging of Ca Dynamics in High-Ca Organelles. Cell Chem Biol. 2016 Jun 1. pii: ...

  4. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dLight1.1 Syn dLight1.1 none Constitutive 1 Tian 111067 pAAV-CAG-dLight1.1 CAG dLight1.1 none Constitutive...pAAV-hSyn-dLight1.2 Syn dLight1.2 none Constitutive 1, 5 Tian 125560 AAV-CAG-dLight1.3b CAG dLight1.3b none...pAAV-syn-dLight1.3b Syn dLight1.3b none Constitutive 9 Tian 125707 pAAV-CAG-RdLight1 CAG RdLight1 none Constitutive...Dopamine Sensors dLight RdLight GRAB_DA GRAB_rDA GRAB_gDA Serotonin Sensors psychLight iSeroSnFR GABA Sensors...187179 pAAV_hSyn1_nLightG Syn nLightG none Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR...Constitutive 9 Tian 125708 pAAV-hSynapsin1-RdLight1 Syn RdLight1 none Constitutive 9 Tian 113049 pAAV-hSyn-GRAB_DA1m...none Cre dependent 9 Tian 163909 pAAV_hSynapsin_psychLight2 Syn psychLight2 none Constitutive 9 Tian GABA...

  5. Antibody Guide

    Type
    Collection
    ...the Fab fragments contain highly variable regions, shown in light blue and light orange, that bind to a ...are light-sensitive molecules which emit a specific wavelength of light after excitation by light of a...pair the brightness of the fluorophore with the abundance of the antibody target. If a bright fluorophore...molecular tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as...-efficient way to produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies...protein chains: two large heavy chains and two small light chains. These four domains come together to form... to be produced in a single individual, giving a high probability that an antibody will be able to recognize...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ... of interest. Tight TRE promoter or P Tight : reduced background expression and tighter binding of Tet-Advanced...gene of interest. tTA-Advanced Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled...systems and how to use them, or skip ahead to view highlighted Tet plasmids . Figure 1: Tet-regulated expression... Left: natural TetR mechanism; center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled... level of doxycycline can access the brain so a highly sensitive transactivator may be needed. Or, when...effector for tet-responsive systems. Dox binds with high affinity to TetR and derivatives like rTetR, rtTA...know whether your serum is tetracycline-free. Highlighted Plasmids Browse the tables below to find popular...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Excitation and Emission maximum are listed in nm. Brightness is the product of exctinction coefficient and...low concentration but are prone to dimerization at high concentration, so this is noted here. Proteins that...Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 ...Top Cyan Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Aquamarine ...Top Green Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids iLOV 447 497... Yellow Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids EYFP 513 527... Orange Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids mKusabira-Orange...

  8. Zebrafish Plasmid Collection

    Type
    Collection
    ...Plasmids and Resources Plasmid Tools Collection Highlights Other Resources The zebrafish ( Danio rerio )...large populations that are amenable to genetics, high-throughput screening and non-invasive live imaging..., and drug discovery and development. Here we highlight some of the plasmids and tools available at Addgene... zebrafish gene or sequence . The table below highlights plasmids that contain tools for use in zebrafish...Plasmid Description Gene/Insert PI Return to top Highlights of Zebrafish Tools in our Collection CRISPR Please...control of gene expression in zebrafish using red light. LipoGlo - Steven Farber Lab. A reporter system ...lipoprotein abundance, size, and localization in high-throughput. MAZERATI platform - Leonard Zon Lab....

  9. CRISPR Guide

    Type
    Collection
    ...joining (NHEJ) pathway and the less efficient but high-fidelity homology directed repair (HDR) pathway....also enhanced specificity by engineering Cas9 into high fidelity Cas9s (hfCas9) . There is currently no ..., which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from Faecalibaculum... downstream of the target site (termed left and right homology arms). The length of each homology arm ...available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...strands Prime editing and base editing both offer high efficiency and low indel formation compared to HDR...favor insertion in the T-RL direction, with the right homology end closest to the target site. Type V-...

  10. Bacterial Expression Systems

    Type
    Collection
    ...constitutively express your gene of interest at high levels. Perhaps high expression leads to slow bacterial growth...pREDawn-AmpR-MCS FixK2 Red light (660 nm) Escherichia coli Heikki Takala 31395 pJT118 PcpcG2 Green Light (532 nm) Escherichia...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the ...Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable Fluorescent Proteins... be controlled by a variety of small molecules, light, and temperature, in different bacterial systems...Escherichia coli Dominic Esposito 43796 pDawn FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940...coli Cheryl Arrowsmith 43795 pDusk pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092...

  11. Optogenetics AAV Preps

    Type
    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... to control cell activity in living tissue with light. Optogenetics is typically used in neuroscience ...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent...pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) GCaMP6m Constitutive ...Deisseroth 137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...

  12. Plan Your Experiment

    Type
    Collection
    ...for a given experiment. In the table below, we highlight the most common categories of genetic manipulations...joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity Dual-nickase...editors Editing efficiency can be low, but generally higher than HDR pegRNA optimization can be challenging...location. Positioning your chosen Cas enzyme at the right spot within the genome relies on proper gRNA design...design a donor DNA to have the appropriate left and right homology arms. See Addgene's HDR blog post for more...guides that target closer to the edit site yield higher editing efficiencies. The template sequences can...specificity, consider using an engineered Cas enzyme with higher fidelity. In addition to off-target activity, it...

  13. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 5 amino acid linker V17V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 17 amino acid linker V32V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting of three Venus fluorescent...amino acid linker, respectively VVVV Anisotropy/brightness standard consisting of four Venus fluorescent...amino acid linker, respectively VVVVV Anisotropy/brightness standard consisting of five Venus fluorescent...connected via 5 amino acid linkers VVVVVV Anisotropy/brightness standard consisting of six Venus fluorescent ...

  14. Antibody Plasmid Collection

    Type
    Collection
    .... In addition to being useful for our bodies to fight off intruders, antibodies are also extremely useful...to researchers in a lab. When an antibody binds tightly to a specific antigen, scientists can use it to...and Expressing Antibodies The following table highlights plasmid collections that can be used to create...Article PI One-step assembly of antibody heavy- and light-chain DNAs into a single mammalian expression vector...Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright pET-30-based vector dedicated to efficient scFv...Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...

  15. Promega Plasmid Collection

    Type
    Collection
    ...assays use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...19.1 kDa and exceptionally bright and stable luciferase that enables highly sensitive detection at endogenous...CRISPR-mediated gene editing outcomes, and conducting high-throughput screening in drug discovery. NanoLuc ...interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions HaloTag (Link ...with LgBiT for bioluminescent assays or with the high-affinity anti-HiBiT antibody for additional detection...together to form an active enzyme and generate a bright luminescent signal in the presence of a substrate...

  16. Plant Plasmids and Resources

    Type
    Collection
    ...collections for use in plants. The tables below highlight plasmids spanning a wide variety of molecular ...The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, ... Li. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and...Return to top Empty Backbones The table below highlights plasmids that can be used as empty backbones ...Return to top Fluorescent Proteins The table below highlights plasmids that feature fluorescent or luminescent...

  17. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...iRFP variants have been shown to have high effective brightness and allow for single or multicolor imaging...near or above 650 nm as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background...iRFPs can be ‘turned on’ by non-phototoxic far-red light and are used for spatially selective imaging of ...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

  18. Optogenetics Plasmid Collection

    Type
    Collection
    ...proteins can also be controlled by light, generally by utilizing a light sensitive protein from cryptochromes...plasmids encode proteins that are controlled by light. Many of these plasmids encode for microbial opsins...archaebacteria, that can be opened and closed in response to light. These proteins can be expressed in specific neurons...membrane channel that opens in response to blue light and allows cations to flow into the cell. Variants...cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as...or search the table below for plasmids that use light to control a protein or cellular activity. For example...

  19. Luciferase Plasmid Collection

    Type
    Collection
    ...Expression Constructs Reporter Constructs Other Highlights You may also like... Fluorescent Proteins Blog...and detection wavelengths. Firefly luciferase is brighter than Renilla luciferase, but is more susceptible... applications. NanoLuc® is small (19 kDa), very bright, and ATP-independent, and a secreted version of...mutant Rluc8, which has increased stability and light output Sanjiv Sam Gambhir 104587 pHIV-iRFP720-E2A-Luc...Description Gene/Insert PI Return to Top Other Highlighted Tools Modified/Novel Luciferases Engineered variants...use the substrate Akalumine with significantly brighter signal and emission in the near-infrared range...

  20. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin mApple Michael Davidson 55165 mCherry-ZO1-C-14 Tight Junctions ...vesicles Clathrin, light polypeptide mRFP Ari Helenius 70217 pEGFP-GFP11-Clathrin light chain Clathrin-coated...cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse or search the ...Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens-1 GFP Alan Fanning *Fusions...-coated vesicles Clathrin light chain GFP Bo Huang 54040 mEmerald-Clathrin-15 Clathrin-coated vesicles...Clathrin-LCa-EYFP Clathrin-coated vesicles Clathrin, light polypeptide EYFP Xiaowei Zhuang 14437 mRFP-Rab5 ...
Showing: 1 - 20 of 88 results