New Norepinephrine Indicators: nLightG and nLightR
Type
Blog Post
Published
Dec. 12, 2023, 2:15 p.m.
... nLightR. A, Structural models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita...indicators: nLightG and nLightR (Kagiampaki et al., 2023).
Figure 1: Properties of nLightG and nLightR...al., 2023.
nLight
nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...norepinephrine. nLight is 28-fold more selective than GRABNE, with both nLightG and nLightR showing significantly...experimental design.
Find nLight plasmids here!
Next steps
While nLightG and nLightR greatly expand the toolbox... both nLightG and nLightR, and may soon have a red tool which matches the sensitivity of nLightG.
Many...images of HEK293T cells and neurons expressing nLightG or nLightR before/after application of NE (10 μM) and...