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pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

Plasmid

#187727

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

Plasmid

#187728

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT_C874only

Plasmid

#187711

Purpose

Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 with mutations (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491; mutations C702A, C776…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ1,2-linker

Plasmid

#187714

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_Δ2,3-linker

Plasmid

#187715

Purpose

Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-NFKB1-Brd 61

Plasmid

#219040

Purpose

Transcription factor NFKB1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

NFKB1 (NFKB1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-ELF4-Brd 47

Plasmid

#219028

Purpose

Transcription factor ELF4 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

ELF4 (ELF4 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-FOSB-Brd 482

Plasmid

#219381

Purpose

Transcription factor FOSB with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

FOSB (FOSB Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-ETV2-Brd 475

Plasmid

#219376

Purpose

Transcription factor ETV2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-JUND-Brd 430

Plasmid

#219345

Purpose

Transcription factor JUND with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

JUND (JUND Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-MBD2-Brd 417

Plasmid

#219336

Purpose

Transcription factor MBD2 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

MBD2 (MBD2 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-ZXDC-Brd 441

Plasmid

#219350

Purpose

Transcription factor ZXDC with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

ZXDC (ZXDC Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-SOX17-Brd 435

Plasmid

#219348

Purpose

Transcription factor SOX17 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-NR2F1-Brd 436

Plasmid

#219349

Purpose

Transcription factor NR2F1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

NR2F1 (NR2F1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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SFFV-RUNX1T1-Brd 496

Plasmid

#219390

Purpose

Transcription factor RUNX1T1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

RUNX1T1 (RUNX1T1 Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-TBX1-Brd 410

Plasmid

#219333

Purpose

Transcription factor TBX1 with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

TBX1 (TBX1 Human)

Use

Lentiviral

Mutation

Codon optimized

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SFFV-LTF-Brd 330

Plasmid

#219260

Purpose

Transcription factor LTF with a specific barcode assigned.

Depositor

Filipe Pereira

Article

Kurochkin et al Cell Syst. 2026 Jan 14, https://doi.org/

Insert

LTF (LTF Human)

Use

Lentiviral

Promoter

The spleen focus-forming virus (SFFV) promoter

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge