We narrowed to 143,240 results for: ins

1. pSN667

   Plasmid

   #219963

   Purpose

   Expressed sfGFP fused human KIF1Bß(1-721) in insect cells

   Depositor

   Shinsuke Niwa

   Insert

   codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
   

   Use

   Tags

   sfGFP::2xStrepII tag

   Expression

   Insect

   Mutation

   encodes 1-721

   Promoter

   polyhedrin

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSN668

   Plasmid

   #219962

   Purpose

   Expressed sfGFP fused human KIF1Bß (1770 a.a. isoform) in insect cells

   Depositor

   Shinsuke Niwa

   Insert

   codon optimized human KIF1Bß (1770a.a. isoform) (KIF1B Human)
   

   Use

   Tags

   sfGFP::2xStrepII tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-IDR4-mCh-Cry2WT-NLS

   Plasmid

   #221920

   Purpose

   Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR4 (aa 662-787) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR4 region (aa 662-787); insert…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CFID-mCh-Cry2WT-NLS

   Plasmid

   #221923

   Purpose

   Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP CFID (aa 1892-2441) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the CBP-FUS interaction domain (CFID…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-IDR3-mCh-Cry2WT-NLS

   Plasmid

   #221919

   Purpose

   Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR3 (aa 441-587) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR3 region (aa 441 - 587); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-AL-mCh-Cry2WT-NLS

   Plasmid

   #221924

   Purpose

   Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP AL (aa 1529-1607) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the autoregulatory loop (AL) (aa 152…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-IDR7-mCh-Cry2WT-NLS

   Plasmid

   #221922

   Purpose

   Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR7 (aa 2109-2434) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR7 region (aa 2109 - 2434); in…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-IDR6-mCh-Cry2WT-NLS

   Plasmid

   #221921

   Purpose

   Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR6 (aa 1856-2090) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR6 region (aa 1856-2090); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-IDR1-mCh-Cry2WT-NLS

   Plasmid

   #221918

   Purpose

   Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR1 (aa 72-175) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR1 region (aa 72 - 175); inser…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. RT-5

    Plasmid

    #220442

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (puf-3 Nematode)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Efg1-ΔDBD-A51P/A56P

    Plasmid

    #216400

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51 and 56 to Proline, and deleted…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Efg1-ΔDBD-Q443P

    Plasmid

    #216401

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Glutamine 443 to Proline, and deleted ami…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAT_41BBICD

    Plasmid

    #197097

    Purpose

    This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for the intracellular domain of 4-1BB.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMAT_CD28ICD

    Plasmid

    #197098

    Purpose

    This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

    Depositor

    Wendell Lim

    Article

    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

    Insert

    This plasmid contains the coding sequence for the intracellular domain of CD28.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMS77

    Plasmid

    #215681

    Purpose

    Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-bullfrog saxiphilin

    Plasmid

    #140595

    Purpose

    protein expression

    Depositor

    Dan Minor

    Article

    Yen et al Sci Adv. 2019 Jun 19;5(6):eaax2650. doi:

    Insert

    saxiphilin bullfrog
    

    Use

    Tags

    GFP-His10

    Expression

    Insect

    Mutation

    Deletion of M20 - please see depositor comment

    Promoter

    polyhedrin promoter

    Available since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MsACR1-pmCerulean3-N1

    Plasmid

    #204958

    Purpose

    Expression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKG-GW2-MaUPPS-B

    Plasmid

    #203175

    Purpose

    Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Insert

    MA_4402
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKG-GW1-MaUPPS-A

    Plasmid

    #203174

    Purpose

    Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Insert

    uppS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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