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pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236376

Purpose

Overexpression of HyperdFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdFnCas12a

Use

CRISPR and Lentiviral

Mutation

E184R, T279R, D340R, D409R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xHSPA1A array

Plasmid

#236377

Purpose

Expression of array of 48 crRNAs targeting HSPA1A

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xHSPA1A crRNA array (HSPA1A Human)

Use

CRISPR and Lentiviral

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for HCT116

Plasmid

#238245

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.0-T2A-EGFP

Plasmid

#216488

Purpose

Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.0

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_EGFP-BRD4-NUT-KS-FtoG

Plasmid

#238256

Purpose

For overexpression of EGFP-BRD4-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-BRD4-NUT-KS-FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoG

Plasmid

#238292

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS-FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS

Plasmid

#238290

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A

Plasmid

#238289

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10

Plasmid

#238284

Purpose

For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-GFP-NUP98-DDX10

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-NPM1-gRNA for HCT116

Plasmid

#238249

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS-FtoG

Plasmid

#238297

Purpose

For RNA injection of Nanog-mNG-KS-FtoG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS-FtoG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-HP1a-repair-template

Plasmid

#238248

Purpose

For knock-in msfGFP to HP1a locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

HP1a

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-NUP98-KDM5A-KS-FtoA

Plasmid

#238295

Purpose

For overexpression of EGFP-NUP98-KDM5A-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-NUP98-KDM5A-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS

Plasmid

#238285

Purpose

For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-GFP-NUP98-DDX10-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoA

Plasmid

#238291

Purpose

For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

EGFP-IRES-NUP98-KDM5A-KS-FtoA

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-linker-KS-FtoA

Plasmid

#237687

Purpose

For overexpression of 52K-GFP-linker-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-linker-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold-CCR5TC

Plasmid

#231784

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mVenus-CCR5TC

Plasmid

#231783

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mVenus

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mVenus-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2t-CCR5TC

Plasmid

#231786

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2t

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2t-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Puro-DEST (JDW 925)

Plasmid

#229808

Purpose

A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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