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Plasmid#179406PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1_MBDDepositorInsertMBD1_MBD
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pv7_CBX1_2xChromo_noEGFP
Plasmid#179395PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo_noEGFPDepositorInsertCBX1_2xChromo_noEGFP
UseTagsAviTagExpressionMammalianMutationPromoterCAGGSAvailable sinceAug. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_Syn_Xph18_eGFP_CCR5TC
Plasmid#187443PurposeEncodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph18
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_Syn_Xph15_eGFP_CCR5TC
Plasmid#187442PurposeEncodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph15
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EGFP_Nick_Dual_sgRNA
Plasmid#178095PurposeControl vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EGFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_eGFP
Plasmid#136095PurposeFP for C-term fusion with CDS12DepositorInsertCTAG_eGFP(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12_eGFP
Plasmid#136085PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_eGFP(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_18bp
Plasmid#174852PurposeExpress forward pegRNA mediating 991-bp deletion and 18-bp insertionDepositorInsertHEK3_pegF_18bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
HEK3_pegF_44bp
Plasmid#174854PurposeExpress forward pegRNA mediating 991-bp deletion and 44-bp insertionDepositorInsertHEK3_pegF_44bp insertion
UseTagsExpressionMammalianMutationPromoterU6Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
DDX41_p.L237F_p.P238T_EGFP
Plasmid#175845PurposeExpresses DDX41 with mutationDepositorInsertDDX41 (DDX41 Human)
UseTagsEGFPExpressionMammalianMutationc.711G>T, p.L237F and c.712C>A, p.P238TPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.EFS.eGFP.pre
Plasmid#169276PurposeConstitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54_mEGFP494(-6)
Plasmid#163434PurposeMammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494DepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-9b)
Plasmid#163420PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-8b)
Plasmid#163418PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW8_yEGFP
Plasmid#110229PurposeCre/Lox C-terminal tagging construct encoding codon-optimized green fluorescent proteinDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.P622S
Plasmid#81721PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationP622SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.G719D
Plasmid#81708PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationG719DPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.R222L
Plasmid#82922PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationR222LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.S645C
Plasmid#82853PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationS645CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_EGFR_p.K754E
Plasmid#82843PurposeGateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.DepositorInsertEGFR (EGFR Human)
UseGateway entry vectorTagsExpressionMutationK754EPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only