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Showing: 481 - 500 of 1043 results

  1. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  2. pSAH71 - [AWAp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200345
    Purpose
    mScarlet BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [AWAp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00003856, odr-10
    Available since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200345-plasmid-map-sequence-id-396807

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  6. pJRM3 - [BAGp | mScarlet | tbb-2 UTR]

    Plasmid
    #204616
    Purpose
    mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Article
    AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791
    Insert
    [BAGp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    204616-plasmid-map-sequence-id-408794

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  7. Double UP mNeon to mScarlet Rac1-N17

    Plasmid
    #125137
    Purpose
    Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).
    Depositor
    Erik Dent
    Article
    Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d
    Insert
    mNeon/mScarletP2A Rac1-N17
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Removed Not1 site in mScarlet. 17th AA in Rac1 ch…
    Promoter
    CAG
    Available since
    May 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    125137-plasmid-map-sequence-id-244754

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  8. pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200335
    Purpose
    mScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [ASELp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001534, gcy-7
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200335-plasmid-map-sequence-id-397589

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  11. pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200359
    Purpose
    mScarlet BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [AWCp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00012770, srt-47
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200359-plasmid-map-sequence-id-397601

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  13. pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200327
    Purpose
    mScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [DDp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00007304, ttr-39
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    200327-plasmid-map-sequence-id-397610

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  15. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  18. pCRISPaint-mScarlet-F-Ova-PuroR [M1G]

    Plasmid
    #171813
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-PuroR
    Use
    Gene tagging
    Tags
    Expression
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Promoter
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171813-plasmid-map-sequence-id-338044

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Showing: 481 - 500 of 1043 results