We narrowed to 1,631 results for: prep

1. pDONR221-PARP7

   Plasmid

   #184474

   Purpose

   For gateway cloning of PARP7

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP7 (TIPARP Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221-PARP15

   Plasmid

   #184471

   Purpose

   For gateway cloning of PARP15

   Depositor

   Karla Feijs

   Article

   Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

   Insert

   PARP15 (PARP15 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

   Plasmid

   #225704

   Purpose

   Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1A

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-MCS-3AID-HA-2A-mCherryBSD

   Plasmid

   #225700

   Purpose

   Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Tags

   T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1A

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

   Plasmid

   #225695

   Purpose

   Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

   Plasmid

   #225690

   Purpose

   Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   rTetR-ZNF10 KRAB
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xFLAG and T2A-mTurquoise

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

   Plasmid

   #222990

   Purpose

   Bacterial expression of iGeoCas9(C1) construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

   Insert

   His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
   

   Use

   CRISPR

   Tags

   CL7, His, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX-SFFV_NKX2-1_Short_V5-GFP

   Plasmid

   #221500

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an SFFV promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX-EF1a_NKX2-1_CdTag-HA

   Plasmid

   #221493

   Purpose

   NKX2-1 short isoform lentiviral overexpression of C-terminal dTag fusion

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_NKX2-1_DelCTerm_1-223AA

    Plasmid

    #221476

    Purpose

    Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Deletion of 224-371AA C-terminal domain

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR223_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221475

    Purpose

    Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223_NKX2-1_HD_160-223AA

    Plasmid

    #221477

    Purpose

    Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Deletion of 1-159AA N-terminal and 224-371AA C-te…

    Promoter

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX-EF1a_NKX2-1_DelNTerm_160-371AA

    Plasmid

    #221487

    Purpose

    NKX2-1 N-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 1-159AA N-terminal domain

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX-hPGK_NKX2-1_Short_V5-GFP

    Plasmid

    #221498

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an hPGK promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX-EF1a_NKX2-1_DelCTerm_1-223AA

    Plasmid

    #221488

    Purpose

    NKX2-1 C-terminal truncation mutation lentiviral overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Deletion of 224-371AA C-terminal domain

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSCL452

    Plasmid

    #219502

    Purpose

    Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSCL390

    Plasmid

    #219500

    Purpose

    Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
    

    Use

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-GAL10

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCL391

    Plasmid

    #219501

    Purpose

    Express Ade2 and Faa1 editrons from a single Gal7 promoter, separated by a Csy4 recognition sequence

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Eco1 editing ncRNAs and Cas9 gRNAs to edit ADE2 and FAA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Efg1-ΔDBD-A51P/A56P

    Plasmid

    #216400

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56 to proline mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-A51P/A56P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Alanine 51 and 56 to Proline, and deleted…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Efg1-ΔDBD-Q443P

    Plasmid

    #216401

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Q443 to proline mutation

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Q443P
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Glutamine 443 to Proline, and deleted ami…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge