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pME_G_0_1_005_5C1SF

Plasmid

#235789

Purpose

5' Connector 1 Short Forward in the M1 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

5' Connector 1 Short Forward

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ABCB10-eYFP

Plasmid

#206038

Purpose

Express YFP in mitochondria

Depositor

Lufang Zhou

Article

Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

Insert

EYFP

Tags

ABCB10 mitochondrial leading sequence

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_004_Amp/ColEI_sfGFP

Plasmid

#235970

Purpose

Ampicillin/ColEI backbone with sfGFP placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al bioRxiv 2024.05.08.593163

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-CaMKII-EGFP-T2A-FlipO

Plasmid

#232791

Purpose

AAV2 vector containing EGFP and FlipO sequences separated by T2A peptide under the control of CaMKIIA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

CaMKII-EGFP-T2A-FlipO

Use

AAV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-roxSTOProx-NaChBac-TdTomato

Plasmid

#236253

Purpose

Dre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Promoter

hSyn

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-FLEX-hM4D(Gi)-mCherry

Plasmid

#232782

Purpose

AAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

hM4D(Gi)-mCherry

Use

AAV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-SP5

Plasmid

#232653

Purpose

transient expresses SP5 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP5 (SP5 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-SP2

Plasmid

#232652

Purpose

transient expresses SP2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

Insert

SP2 (SP2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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PAF23

Plasmid

#223171

Purpose

Expresses yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

green fluorescent protein

Expression

Yeast

Promoter

Saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PAF24

Plasmid

#223172

Purpose

Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

Copper Transporter

Tags

StreptagII and yeGFP

Expression

Yeast

Promoter

saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGH643

Plasmid

#230834

Purpose

hu6-BsmBI-MS2_sgRNA-Ef1a-puroR-T2A-mCherry-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH394

Plasmid

#230851

Purpose

Ef1a-rAPOBEC1-dCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-dCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH398

Plasmid

#230854

Purpose

Ef1a-rAPOBEC1-n'Cas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-n'Cas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH401

Plasmid

#230852

Purpose

Ef1a-rAPOBEC1-nCas9-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

rAPOBEC1-nCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-C9BoNT-A

Plasmid

#202490

Purpose

Expresses the nanobody C9 BoNT/A with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-GFPuv

Plasmid

#202463

Purpose

Expresses GFPuv with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-L1374

Plasmid

#226275

Purpose

Plasmid expressing the SEC18 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-S288C

Plasmid

#226274

Purpose

Plasmid expressing the SEC18 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-NCYC110

Plasmid

#226277

Purpose

Plasmid expressing the SEC18 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SEC18-UWOPS872421

Plasmid

#226276

Purpose

Plasmid expressing the SEC18 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC18 (SEC18 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-S288C

Plasmid

#226266

Purpose

Plasmid expressing the SCT1 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-L1374

Plasmid

#226267

Purpose

Plasmid expressing the SCT1 allele from L-1374, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-NCYC110

Plasmid

#226269

Purpose

Plasmid expressing the SCT1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS313-SCT1-UWOPS872421

Plasmid

#226268

Purpose

Plasmid expressing the SCT1 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SCT1 (SCT1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pGH1110

Plasmid

#223682

Purpose

U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPSt

Plasmid

#203590

Purpose

Base plasmid for Plant STARR-seq terminator libraries.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Expression

Plant

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL194

Plasmid

#222225

Purpose

Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 1/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL195

Plasmid

#222226

Purpose

Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

site 2/Cas9/Eco1 RT

Use

CRISPR

Expression

Yeast

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge