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We narrowed to 5,482 results for: pcDNA3.1+

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Showing: 4981 - 5000 of 5482 results

  2. pHA StreptagII 3C NIS EABR

    Plasmid
    #234988
    Purpose
    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface
    Depositor
    Monica Gonzalez-Magaldi, Daniel Leahy
    Article
    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.
    Insert
    NIS-EABR (SLC5A5 Human)
    Tags
    HA, Streptag-II, HRV 3C site
    Expression
    Mammalian
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    234988-plasmid-map-sequence-id-466789

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  4. pcDNA.3.1.ADRB2-NP

    Plasmid
    #134376
    Purpose
    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    ADRB2-NP (ADRB2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    Aug. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134376-plasmid-map-sequence-id-306396

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  6. pC0048-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q)

    Plasmid
    #103864
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GSGGGGS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103864-plasmid-map-sequence-id-199176

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  7. pAB1676 REPAIR.t1

    Plasmid
    #176319
    Purpose
    CMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176319-plasmid-map-sequence-id-345279

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  10. eiABE

    Plasmid
    #227144
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain was inserted between TadA-8e and eIscBn driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-HMG-D-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227144-plasmid-map-sequence-id-453987

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  11. GANLS-DEVD-BNES

    Plasmid
    #50835
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50835-plasmid-map-sequence-id-76278

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  13. eGFP-proα1(I)

    Plasmid
    #119843
    Purpose
    Expresses mouse Type I procollagen α1 chain (Col1a1) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α1 chain (Col1a1 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119843-plasmid-map-sequence-id-240348

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  16. pcDNA3-sACE2v2.4(732)-IgG1

    Plasmid
    #154106
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available Since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154106-plasmid-map-sequence-id-301643

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  20. eiCBE

    Plasmid
    #227148
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoter
    Depositor
    Dali Li
    Article
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-hA3A*-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227148-plasmid-map-sequence-id-453981

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Showing: 4981 - 5000 of 5482 results