We narrowed to 2,914 results for: GRI;

1. pJC20cdc8.110

   Plasmid

   #99363

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine and Glutamic acid 31 to L…

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-PIAL2M-sim1

   Plasmid

   #67908

   Purpose

   Inducible expression of a mutant of PIAL2M where the SIM interacting motif 1 (VFDL 425-428) was substituted with alanines.

   Depositor

   Andreas Bachmair

   Article

   Tomanov et al Plant Cell. 2014 Nov;26(11):4547-60. doi

   Insert

   PIAL2 (AT5G41580 Mustard Weed)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   E281-A496; VEDL 425-428 AAA

   Promoter

   P-lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pUCmini-iCAP-AAV.MaCPNS1

   Plasmid

   #185136

   Purpose

   non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

   Insert

   Synthetic construct isolate AAV-MaCPNS1 VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3` CC: Hygro – H2B-GFP-N – loxP – mScarlet

   Plasmid

   #219565

   Purpose

   3` circularization cassette to induce Cre-mediated circularization of a genomic region flanked by loxP sites with H2B-GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   HygroR_2A_H2B-GFP-N_loxP_mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNL-CEF-CD79B-RFP

   Plasmid

   #187005

   Purpose

   Expression of human CD79B as RFP fusion protein

   Depositor

   Hermann Eibel

   Article

   Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

   Insert

   CD79B (CD79B Human)
   

   Use

   Lentiviral

   Tags

   tagRFP

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMALB-ATF4.5

   Plasmid

   #155032

   Purpose

   Fluorescent reporter for ATF4 translation

   Depositor

   Peter van Galen, John Dick

   Article

   van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

   Inserts

   ATF4 5' UTR (Atf4 Mouse)
   ATF4-eGFP fusion ORF
   TagBFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB53x EF1-Dendra2-RPB1Amr

   Plasmid

   #81228

   Purpose

   expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

   Depositor

   Timothée Lionnet

   Article

   Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

   Insert

   Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   N792D alpha amanitin resistance mutation (Bartolo…

   Promoter

   EF1a

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ma-Barnase

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Ma-pREP

   Plasmid

   #197571

   Purpose

   Positive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a ori

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Chloramphenicol resistance gene - 111TAG
   T7 polymerase -TAG
   GFPuv
   M. alvus Pyl-tRNA(6)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   111TAG and 8TAG/112TAG

   Promoter

   AraC, T7, cat promoter (constitutive), and lpp (c…

   Available since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMALB-ATF4.12

    Plasmid

    #155033

    Purpose

    Fluorescent reporter for ATF4 translation, Negative Control

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    uORF1 start codon mutated

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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