We narrowed to 159,557 results for: addgene

1. pCMV-T7-ABE8.20m-nSpCas9-VRQR-P2A-EGFP (KAC1166)

   Plasmid

   #185918

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-VRQR-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICH-roGFP2-ORP1

   Plasmid

   #191677

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   redox(ro)GFP2-ORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-NEAT1pr_v2

   Plasmid

   #97082

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. IRES-H2B-YFP-DD

   Plasmid

   #96893

   Purpose

   Reporter to monitor translation of EMCV IRES mRNA

   Depositor

   Tobias Meyer

   Article

   Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

   Insert

   IRES-H2B-Venus-DD
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACAGW-oChiEFtdTomato-AAV

   Plasmid

   #32846

   Purpose

   AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

   Depositor

   Rory McQuiston

   Article

   Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

   Insert

   oChiEF
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAGG

   Available since

   March 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH-chl-roGFP2-synORP1

   Plasmid

   #191701

   Purpose

   Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   chl-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S promoter

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pICH-nuc-roGFP2-synORP1

   Plasmid

   #191759

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-synORP1 for H2O2 detection in the nuclei of plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-synORP1
   

   Use

   Binary agrobacterium ti plasmid

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1 RagB shRNA #1

   Plasmid

   #26627

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagB shRNA (RRAGB Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pRG645_lexO-AscI_LexA-TF_LEU2MX

   Plasmid

   #154813

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5xHRE-MinTK-CRE

    Plasmid

    #141145

    Purpose

    lentiviral expression vector to generate a hypoxia fate-mapping system

    Depositor

    Daniele Gilkes

    Article

    Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

    Insert

    5xHRE-MinTK-CRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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