161,273 results

1. pS548∙CsR

   Plasmid

   #197859

   Purpose

   Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

   Depositor

   Susanne Häußler

   Article

   Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

   Insert

   gRNA scaffold - Tc resistance cassette - incP oriT
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PEM7

   Available since

   Oct. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Bxb1o_nlsFlag

   Plasmid

   #119901

   Purpose

   Expresses Bxb1 integrase in mammalian cells

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   Mammalian codon optimized Bxb1 integrase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS

   Plasmid

   #60904

   Purpose

   The plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUC01

   Plasmid

   #177204

   Purpose

   chromogenic reporter for gene knockout in Streptomyces

   Depositor

   Wenjun Guan

   Article

   Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1

   Insert

   SaindC
   

   Use

   Synthetic Biology

   Tags

   NONE

   Expression

   Bacterial

   Mutation

   NONE

   Promoter

   TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. MAC-TFRC

   Plasmid

   #172420

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

   Insert

   Transferrin receptor protein 1 (TfR) (CD antigen CD71) (TFRC Human)
   

   Use

   Tags

   MAC-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AbVec2.1-mIglc2

   Plasmid

   #127156

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Iglc2 (Iglc2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AbVec2.0-mIghg2b

   Plasmid

   #127155

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Ighg2b (Ighg2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. IKK-2 WT

    Plasmid

    #11103

    Depositor

    Anjana Rao

    Article

    Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

    Insert

    IKK-2 (IKBKB Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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