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Plasmid#149445PurposeExpression of MIF H62F in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62FPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62A
Plasmid#149443PurposeExpression of MIF H62A in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62APromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF H62Y
Plasmid#149442PurposeExpression of MIF H62Y in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationH62YPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF Y99G
Plasmid#149441PurposeExpression of MIF Y99G in E. coli for protein productionDepositorInsertMIF (MIF Human)
UseTagsExpressionBacterialMutationY99GPromoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-BTRC-MGMT_2
Plasmid#136416PurposeLentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(BTRC)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP63.1.0-gDNA
Plasmid#132432PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTP63 (TP63 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c025
Plasmid#139762PurposeProtein expression/GST pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c023
Plasmid#139760PurposeProtein expression/GST pull-downDepositorInsertTBXT, DNA-binding domain, WT (TBXT Human)
UseTagsHis6-GST-TEVExpressionBacterialMutationContains only amino acids E41-D225PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c022
Plasmid#139759PurposeProtein expression/crystallographyDepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
UseTagsHis6-SUMOExpressionBacterialMutationG177D; contains only amino acids E41-A214PromoterT7Available sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP63-donor
Plasmid#132477PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTP63 (TP63 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR-Hu_TTK1(WT)
Plasmid#136354PurposeHuman TTK (Mps1) cDNA in Gateway pENTRDepositorInsertTTK (TTK Human)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pE4F1.1.0-gDNA
Plasmid#132451PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertE4F1 (E4F1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDLX4.1.0-gDNA
Plasmid#132437PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertDLX4 (DLX4 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-EGFPKir7.1
Plasmid#107181PurposeLentiviral particle generationDepositorInsertKCNJ13 (KCNJ13 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterEF1AAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
6DXH (USP05)
Plasmid#119968PurposeBacterial expression for structure determinationDepositorInsertUSP05 (USP5 Human)
UseTags6xHis-TEVExpressionBacterialMutationcontains amino acids 171-290PromoterT7Available sinceJan. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pZNF174-donor
Plasmid#112301PurposeCRISPR donor plasmid to tag human transcription factor ZNF174 with GFPDepositorInsertZNF174 homology arms flanking EGFP-IRES-Neo cassette (ZNF174 Human)
UseCRISPRTagsExpressionMutationrs37813, rs39729PromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF257-donor
Plasmid#112294PurposeCRISPR donor plasmid to tag human transcription factor ZNF257 with GFPDepositorInsertZNF257 homology arms flanking EGFP-IRES-Neo cassette (ZNF257 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF586-donor
Plasmid#112324PurposeCRISPR donor plasmid to tag human transcription factor ZNF586 with GFPDepositorInsertZNF586 homology arms flanking EGFP-IRES-Neo cassette (ZNF586 Human)
UseCRISPRTagsExpressionMutationhg38:19:57779607:T>APromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF134-donor
Plasmid#112320PurposeCRISPR donor plasmid to tag human transcription factor ZNF134 with GFPDepositorInsertZNF134 homology arms flanking EGFP-IRES-Neo cassette (ZNF134 Human)
UseCRISPRTagsExpressionMutationhg38:19:57621700:C>TPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF696.1.0-gDNA
Plasmid#113773PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF696DepositorInsertZNF696 (ZNF696 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only