We narrowed to 14,607 results for: pET

1. CSK_HUMAN_D0

   Plasmid

   #79683

   Purpose

   This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSK (CSK Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. OPEN-A*24:02

   Plasmid

   #235133

   Purpose

   E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. OPEN-HLA-A*01:01

   Plasmid

   #235134

   Purpose

   E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*01:01 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. WT USP7 UBL-12

   Plasmid

   #227230

   Purpose

   Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 535-775

   Promoter

   T7-promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG8Ha-His-EWSR1-359-549

   Plasmid

   #195868

   Purpose

   Expresses the RRM-RG2-ZnF (359-549) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWS 359-549 (EWSR1 Human)
   

   Use

   Tags

   His8-linker-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSG086

   Plasmid

   #214263

   Purpose

   PaFtsH2H1-link-32-6xHis: PaftsH2(ftsH2_F124-V154del_ins ftsH1_M120-T151)-6xHis cloned in XbaI/NheI site in pET-28a(+)

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   Hybrid PaFtsH protease PaFtsH2H1-link-32 of Pseudomonas aeruginosa SG17M
   

   Use

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   substitution of 31 amino acids of FtsH2 (F124RRFA…

   Promoter

   T7 promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACB120

   Plasmid

   #222213

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with five mutations (K10N, S11R, W15C, G76V, G258D) and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(K10N, S11R, W15C, G76V, G258D)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Contains five amino acid mutations: K10N, S11R, W…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACB143

   Plasmid

   #222214

   Purpose

   For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   vanB(A24P)
   

   Use

   Tags

   N-terminal, thrombin-cleavable 6x His tag

   Expression

   Bacterial

   Mutation

   Changed Alanine 24 to Proline

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACB111

   Plasmid

   #222210

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA(G76V)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed glycine 76 to valine

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACB112

    Plasmid

    #222211

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(S11R)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Serine 11 to Arginine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACB113

    Plasmid

    #222212

    Purpose

    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag

    Depositor

    Gregg Beckham

    Article

    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

    Insert

    fghA(W15C)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Changed Tryptophan 15 to Cysteine

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FENEF-Sensor

    Plasmid

    #208930

    Purpose

    in vitro p38-Sensor for luminescent detection of p38-activity

    Depositor

    Attila Reményi

    Article

    Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

    Insert

    FENEF sequence from human ATF2 with phospho site (ATF2 Human)
    

    Use

    Tags

    HISx6, MBP, and Smbit

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1-A11(2-196, G175R)-Strep

    Plasmid

    #196211

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G175R

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-A11(2-196, G189E)-Strep

    Plasmid

    #196212

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Use

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Mutation

    G189E

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GB1-CEP55(160-217, S215C)

    Plasmid

    #186793

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CEP55(160-217, S215C)

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-CEP55(160-217, S215C) (CEP55 Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    S215C

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

    Plasmid

    #199242

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    S191C, I225A, L228A, W231A

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GB1-ΔN-CHMP4B(S184C)

    Plasmid

    #180026

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4B(121-224, S184C).

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-CHMP4C(121-224, S184C) (CHMP4B Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    S184C

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSN-A112C-1A32

    Plasmid

    #191241

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Use

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSN-A112C-1A31

    Plasmid

    #191242

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Use

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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