Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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AA370

Plasmid

#215988

Purpose

Fragmid fragment: (N' terminus) attenuate expression

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA1

Plasmid

#201592

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ENO1_sgRNA2

Plasmid

#201593

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

ENO1 (ENO1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BJ.1-AVI

Plasmid

#196620

Purpose

Expresses SARS-CoV-2 S2P protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-BJ.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEGPN-coBCL2L1-G94A

Plasmid

#188697

Purpose

express codon optimized BCL-XL drug resistant mutant

Depositor

Lewis Cantley

Insert

coBCL2L1-G94A (BCL2L1 Human)

Use

Lentiviral

Mutation

codon optimized, G94A

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGPN-coBCL2L1-F97C

Plasmid

#188696

Purpose

express codon optimized BCL-XL drug resistant mutant

Depositor

Lewis Cantley

Insert

coBCL2L1-F97C (BCL2L1 Human)

Use

Lentiviral

Mutation

codon optimized, F97C

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#1/Cre

Plasmid

#173659

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#2/Cre

Plasmid

#173660

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-BA.2-AVI

Plasmid

#184531

Purpose

Expresses SARS-CoV-2 S2P protein from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-BA.2 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19I, L24-, P25-, P26-, A27S, G142D, V213G, G339D…

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-C.37-AVI

Plasmid

#181696

Purpose

Expresses SARS-CoV-2 NTD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G75V, T76I, R246N, ∆S247-∆D253,

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-C.37-AVI

Plasmid

#181699

Purpose

Expresses SARS-CoV-2 RBD domain from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452Q, F490S

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-S2P-C.37-AVI

Plasmid

#181693

Purpose

Expresses SARS-CoV-2 S2P protein from the C.37 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-S2P-C.37 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G75V, T76I, R246N, ∆S247-∆D253, L452Q, F490S, D61…

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-L452R-AVI

Plasmid

#176452

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain containing L452R mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-L452R (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-S477N-AVI

Plasmid

#176453

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain containing S477N mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-S477N (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

S477N

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-E484K-AVI

Plasmid

#176454

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain containing E484K mutation with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-E484K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

E484K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L452R-E484Q-AVI

Plasmid

#176445

Purpose

Expresses SARS-CoV-2 RBD domain containing L452R-E484Q mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-L452R-E484Q (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R, E484Q

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-L452R-T478K-AVI

Plasmid

#176446

Purpose

Expresses SARS-CoV-2 RBD domain containing L452R-T478K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-L452R-T478K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

L452R, T478K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-SD1-WA1-AVI

Plasmid

#176448

Purpose

Expresses SARS-CoV-2 RBD-SD1 domain from the WA1 strain with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-SD1-WA1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-G142D-E154K-AVI

Plasmid

#176434

Purpose

Expresses SARS-CoV-2 NTD domain containing G142D-E154K mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-G142D-E154K (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G142D, E154K

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-NTD-AY.1-AVI

Plasmid

#176437

Purpose

Expresses SARS-CoV-2 NTD domain from the AY.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-NTD-AY.1 (S )

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

T19R, T95I, G142D, del156-157, R158G, W258L

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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