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pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215911

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

Flag-HA-SBP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

Plasmid

#215905

Purpose

Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

Depositor

Kumiko Ui-Tei

Publication

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1709, S1332A, S1346A, S1616A, S169…

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBSII-FRT-MCS

Plasmid

#205988

Purpose

Vector containing FRT and FRT-F3 sites

Depositor

Sharon Torigoe

Publication

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Type

Empty backbone

Use

Pcr vector

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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RT-3

Plasmid

#220440

Purpose

Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)

Depositor

Marvin Wickens

Publication

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Promoter

MV IE93 promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Publication

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5Q-GS3

Plasmid

#204746

Purpose

Expression of Cas9 and human H2AX with the K5Q mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5Q (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5Q mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K5R-GS3

Plasmid

#204747

Purpose

Expression of Cas9 and human H2AX with the K5R mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K5R (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K5R mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134L-GS3

Plasmid

#204750

Purpose

Expression of Cas9 and human H2AX with the K134L mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134L (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134L mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134M-GS3

Plasmid

#204751

Purpose

Expression of Cas9 and human H2AX with the K134M mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134M (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134M mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.X_K134A-GS3

Plasmid

#204752

Purpose

Expression of Cas9 and human H2AX with the K134A mutation

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AX K134A (H2AX Human)

Expression

Mammalian

Mutation

H2AX with the K134A mutation

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-BOS-LGP2-Myc

Plasmid

#215874

Purpose

LGP2-FLAG expression vector for overexpression (not Myc tag)

Depositor

Kumiko Ui-Tei

Publication

Takahashi et al Genes (Basel). 2018 Oct 19;9(10):511. do

Insert

LGP2 (DHX58 Human)

Tags

FLAG, 6xHis

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pXOOY

Plasmid

#196449

Purpose

A dual vector for expression of membrane proteins in S. cerevisiae and X. laevis

Depositor

Per Amstrup Pedersen

Publication

Vold et al PLoS One. 2023 Feb 21;18(2):e0281868. do

Type

Empty backbone

Use

Expression in xenopus laevis

Expression

Yeast

Promoter

yeast CYC-GAL and T7 promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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K365-iLid

Plasmid

#188456

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Publication

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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K365-micro

Plasmid

#188457

Purpose

Component of optically-induced motor dimerization system

Depositor

Zvonimir Dogic

Publication

Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

Insert

Kinesin 1-365 (Khc Fly)

Tags

6xHis-MBP-TEV and SspB micro

Expression

Bacterial

Mutation

Kinesin amino acids 1-365

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Publication

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCIneo-lambdaN-HA-HsSmg7_1-633-shRNAres_U

Plasmid

#147645

Purpose

Mammalian Expression of HsSmg7_1-633-shRNAres

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633-shRNAres (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G co…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7_633-1091-siRNAres_U

Plasmid

#147646

Purpose

Mammalian Expression of HsSmg7_633-1091-siRNAres

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_633-1091-siRNAres (SMG7 Human)

Expression

Mammalian

Mutation

two non silent mutations Q651R and V854I compared…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot8_1-240_U

Plasmid

#147648

Purpose

Bacterial Expression of HsNot8_1-240

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsNot8_1-240 (CNOT8 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5_267-1017-V5-SBP-MBP_V

Plasmid

#147762

Purpose

Mammalian Expression of HsSmg5_267-1017

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5_267-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-del133-155-V5-SBP-MBP_V

Plasmid

#147763

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Publication

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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