We narrowed to 10,337 results for: plasmids 101
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Plasmid#64160PurposePhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.DepositorInsertsgRNA1 for pNeurog2-Luciferase reporter
UseCRISPRTagsExpressionMammalianMutationPromoterhuman U6Available SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ATLASsnCre
Plasmid#232351PurposeAnterograde transsynaptic tracer protein to express in presynaptic glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells.DepositorHas ServiceAAV5InsertATLASsnCre
UseAAVTagsALFA-TagExpressionMutationPromoterhSynAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4
Plasmid#27076PurposeNon-integrating (episomal) expression of human OCT3/4DepositorAvailable SinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin S
Plasmid#11251PurposeMammalian expression of human Cathepsin SDepositorAvailable SinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIVM_02
Plasmid#204500PurposeExpression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)DepositorInsertamino acids 54-213 VHL (VHL Human)
UseTags6xHis, TEV, and ThrombinExpressionBacterialMutation0PromoterAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA_J23119-sgRNA
Plasmid#108550PurposeBacterial Target-AID vector with sgRNADepositorInsertsdCas9-PmCDA1
Streptococcus pyogenes sgRNA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9PromoterJ23119 and lambda ORAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway cloningTagsMLSExpressionMutationPromoterAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9 fused to nanobody (anti-beta catenin)
Plasmid#186420PurposeExpression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tagDepositorInsertdCas9-nanobody fusion (anti-beta catenin)
UseCRISPRTags6HisExpressionMutationPromoterAvailable SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKHR8
Plasmid#74625Purposea plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.DepositorInsertsFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
cmlc2::mCherry
UseCre/Lox; Zebrafish expressionTagsExpressionMutationPromoteralpha-crystallin and cmlc2Available SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHOHO2_Hsp82viiA
Plasmid#61708PurposeYeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.DepositorInsertHsp82viiA (HSP82 Budding Yeast)
UseYeast integration vector targeting inserts to the…Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterTEF1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Brp-T2A-QF2w-Hsp70-3XP3-dsRed
Plasmid#141094PurposeTemplate plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.DepositorInsertbruchpilot
UseCRISPRTagsExpressionInsectMutationcoding sequence downstream of CRISPR cut site, sy…PromoterAvailable SinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRTagsExpressionMutationPromoterPtetAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Cas13d-T2A-Citrine
Plasmid#224565PurposeUbiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.DepositorInsertCas13d-T2A-Citrine
UseCRISPRTagsT2A-CitrineExpressionMutationPromoterAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1
Plasmid#164800PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1DepositorInsertsERH CRISPRi gRNA1
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2
Plasmid#164801PurposeFor CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2DepositorInsertsERH CRISPRi gRNA2
puro-T2A-BFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1Alpha and U6Available SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist Amp_2xFlag-dSERT-HDR-donor-HDR
Plasmid#221835PurposeDonor plasmid for inserting 2xFLAG at the beginning of Drosophila SERT coding frameDepositorInsert2xFLAG with dSERT homology arms
UseCRISPRTagsExpressionInsect and MammalianMutationPromoterAvailable SinceAug. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 sfgfp
Plasmid#226371PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertsfGFP
UseTags6x HisTagExpressionBacterialMutationPromotercopA promoter (pCopA) and RBS30 regulationAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK M15 (UUA)
Plasmid#154785Purposeplasmid with single copy of Mma PylT M15 mutant (UUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationUUA anticodon and G10A, G14U, G16U, C20U, C52APromoterh7SKAvailable SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hOCT3/4-shp53-F
Plasmid#27077PurposeIntegration-free (episomal) expression of human OCT3/4 and shRNA against p53DepositorAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only