We narrowed to 42,665 results for: cha

1. PX458-3xHA-en31FnCas9

   Plasmid

   #201949

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en31FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   G1243T, E1369R, E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458-3xHA-en1FnCas9

   Plasmid

   #201947

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en1FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1369R on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

   Plasmid

   #55614

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   C4762 mutated to serine to render HERC2 catalytic…

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLCKO-CMV-TMEM106B-P2A-Blasticidin

   Plasmid

   #237478

   Purpose

   Encodes full length human TMEM106B protein, along with blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B (TMEM106B Human)
   

   Use

   Lentiviral

   Tags

   P2A-Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

   Plasmid

   #238132

   Purpose

   This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

   Plasmid

   #238131

   Purpose

   This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

   Depositor

   Dirk Daelemans

   Article

   Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

   Insert

   TMEM106B
   

   Use

   Lentiviral

   Tags

   Blasticidin-P2A-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458-3xHA-FnCas9ABEmax8.17d

   Plasmid

   #201955

   Purpose

   Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. RPL25-GFP (94)

   Plasmid

   #24037

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

10. RPS1B-GFP (95)

    Plasmid

    #24039

    Depositor

    Michael Rout

    Insert

    RPS1B (RPS1B Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only