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pT7-7 asyn S129W
Plasmid#105744PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein S129W (SNCA Human)
UseTagsExpressionBacterialMutationS129WPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn K32R
Plasmid#105739PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein K32R (SNCA Human)
UseTagsExpressionBacterialMutationK32RPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HECW2 WW domain #1
Plasmid#104244PurposeBacterial expression of WW domain #1 from HECW2DepositorInsertHECW2 WW domain #1 (HECW2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
5WCG (SMYD2)
Plasmid#101268PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertSMYD2 (SMYD2 Human)
UseBaculovirus expressionTags6xHis-TEVExpressionMutationPromoterpolyhedrinAvailable sinceOct. 30, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
2QNR (NEDD5)
Plasmid#101273PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNEDD5 (SEPTIN2 Human)
UseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5tnC/pBSSK+
Plasmid#98119PurposeCMYA5 (NM_153610, nt 11648 to 12279) in pBSSK+ (minimum binding region to titin)DepositorInsertCMYA5 (CMYA5 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 18, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMYA5tnC/pENTR3C
Plasmid#98128PurposeCMYA5 (NM_153610, nt 11648 to 12279) in pENTR3C, Gateway Entry vectorDepositorInsertCMYA5 (CMYA5 Human)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceAug. 3, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDk4-luciferase fragment 4
Plasmid#86663PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated from 880 -644 to -880PromoterCDK4Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment 3
Plasmid#86662PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated from 880 -416 to -658PromoterCDK10Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDk4-luciferase fragment 2
Plasmid#86661PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationgenerated from 880 -201 to -430PromoterCDK9Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
alpha4-nAChR shRNA (Alpha-4.3)
Plasmid#86652Purposeexpression of shRNA targeting CHRNA4DepositorInsertCHRNA4 (alpha4-nAChR) (CHRNA4 Human)
UseRNAiTagsExpressionMammalianMutationPromoterH1Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
R777-E337 Hs.STK3
Plasmid#70621PurposeGateway ORF clone of human STK3 [NM_006281.3] with stop codon (for native or N-terminal fusions)DepositorInsertSTK3 (STK3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E338 Hs.STK3-nostop
Plasmid#70622PurposeGateway ORF clone of human STK3 [NM_006281.3] without stop codon (for C-terminal fusions)DepositorInsertSTK3 (STK3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E300 Hs.RPS6KB2-nostop
Plasmid#70584PurposeGateway ORF clone of human RPS6KB2 [NM_003952.2] without stop codon (for C-terminal fusions)DepositorInsertRPS6KB2 (RPS6KB2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E278 Hs.RHOA-nostop
Plasmid#70562PurposeGateway ORF clone of human RHOA [NM_001664.2] without stop codon (for C-terminal fusions)DepositorInsertRHOA (RHOA Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E058 Hs.EIF4EBP2-nostop
Plasmid#70342PurposeGateway ORF clone of human EIF4EBP2 [NM_004096.4] without stop codon (for C-terminal fusions)DepositorInsertEIF4EBP2 (EIF4EBP2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E060 Hs.EIF4EBP3-nostop
Plasmid#70344PurposeGateway ORF clone of human EIF4EBP3 [NM_003732.2] without stop codon (for C-terminal fusions)DepositorInsertEIF4EBP3 (EIF4EBP3 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E024 Hs.CDK4-nostop
Plasmid#70308PurposeGateway ORF clone of human CDK4 [NM_000075.3] without stop codon (for C-terminal fusions)DepositorInsertCDK4 (CDK4 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
5C0M
Plasmid#69962PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSGF29:JMC01N-E01:C207060 (SGF29 Human)
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits
