We narrowed to 9,135 results for: Pol

1. psicheck-2-Mrpl11-mod

   Plasmid

   #49569

   Purpose

   contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

   Depositor

   Vamsi Mootha

   Article

   Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

   Insert

   Mrpl11-mod
   

   Use

   Luciferase

   Mutation

   T/A mutation in ATG start site

   Available Since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET-NEMOL329P

   Plasmid

   #11969

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMOL329P (IKBKG Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Leucine329 to Proline

   Available Since

   July 13, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pGBW-m4046831

   Plasmid

   #145671

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;Myc

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pKP4010

   Plasmid

   #113336

   Purpose

   Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

   Inserts

   Artificial Transcription Factor (McIsaac et al, 2013)
   Ty3 Integrase
   

   Tags

   3X-FLAG/8X-Gly linker

   Expression

   Yeast

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5 Halo RagB

   Plasmid

   #112753

   Purpose

   express Halo-RagB

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Halo-RagB (RRAGB Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz74_Rubisco_CsoS2_Npeptide_fusion

   Plasmid

   #140840

   Purpose

   Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
   

   Tags

   CsoS2 N-peptide consensus to CbbL C-terminus and …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5A1-Ti

   Plasmid

   #92018

   Purpose

   Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

   Depositor

   Brian Pfleger

   Article

   Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

   Inserts

   TALE targeting lac operator with 3 TEV cleavage sites
   Catalytically Inactive TEV Protease
   

   Use

   TALEN

   Tags

   5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

   Mutation

   S219V, C151A

   Available Since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

   Plasmid

   #46838

   Purpose

   Express cGFP transcript ending in the minimal functional MALAT1 triple helix

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pY71-tet.I.16

   Plasmid

   #133536

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIS1 IMP-1 short UTR

    Plasmid

    #21639

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. psicheck-2-Sfxn3-mod

    Plasmid

    #48170

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of SFXN3 (SFXN3 Human)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

    Plasmid

    #46840

    Purpose

    Express cGFP transcript ending in the wildtype MEN beta triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 GTF2H4

    Plasmid

    #67094

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H4 (GTF2H4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 MAPK13

    Plasmid

    #67077

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    MAPK13 (MAPK13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pIS1 IMP-1 rc

    Plasmid

    #21643

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGST1-GGA1-VHS

    Plasmid

    #44418

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    GGA1-VHS (1-148) (GGA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. FLAG-mPar6a WT

    Plasmid

    #86148

    Purpose

    FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

    Depositor

    Senthil Muthuswamy

    Article

    Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

    Insert

    Par6 (Pard6a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 ATF3

    Plasmid

    #67057

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    M2I

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. p.UTA.2.0 miR 19b-3p

    Plasmid

    #82442

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-19-3p
    

    Mutation

    Complementary region for hsa-miR-19-3p as sensor

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5A7-T

    Plasmid

    #92020

    Purpose

    Expresses TALE targeted to lac operator with one TEV cut site in its N-terminal domain and a second in the C-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with N and C-terminal TEV protease cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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