We narrowed to 10,632 results for: t7

1. pBS-FAK

   Plasmid

   #74478

   Purpose

   This construct was engineered for the purpose of cloning and sequencing Focal Adhesion Kinase.

   Depositor

   J Thomas Parsons

   Article

   Schaller et al Proc Natl Acad Sci U S A. 1992 Jun 1;89(

   Insert

   FAK (PTK2 Chicken)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZS*11-yfp0

   Plasmid

   #53241

   Purpose

   express yellow fluorescent protein (YFP) with strong T7 RBS at a low gene dosage (3-4 copies/cell)

   Depositor

   Philippe Cluzel

   Article

   Subramaniam et al Proc Natl Acad Sci U S A. 2013 Feb 5;110

   Insert

   YFP
   

   Expression

   Bacterial

   Promoter

   PLtetO1

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TAL2417

   Plasmid

   #36818

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   FANCA-TALEN_Right (Fanca Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK220

   Plasmid

   #71123

   Purpose

   Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   pantothenate kinase
   

   Tags

   H6

   Expression

   Bacterial

   Mutation

   Arg175 is mutated to a His

   Promoter

   T7

   Available Since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXG-hXRCC1

   Plasmid

   #52283

   Purpose

   Bacterial expression of human XRCC1 C-terminally fused to GyrA intein and GST

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Insert

   XRCC1 (XRCC1 Human)
   

   Tags

   GyrA-intein-GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCellFree_G03 PPARG

   Plasmid

   #67099

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PPARG (PPARG Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pRC05

   Plasmid

   #114684

   Purpose

   FKBP-TEV-C

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   FKBP-TEV-C
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Sept. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-C. thermophilum LIC

   Plasmid

   #74780

   Purpose

   encodes GST-tagged dynein light intermediate chain from Chaetomium thermophilum

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   dynein light intermediate chain (Chaetomium thermophilum)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST14-SAR1

    Plasmid

    #67388

    Purpose

    Expression of native hSar1 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Promoter

    T7

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TAL2416

    Plasmid

    #36817

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FANCA-TALEN_Left (Fanca Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCellFree_G03 PSMC1

    Plasmid

    #67087

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PSMC1 (PSMC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    E275G, I287V and R294G

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET28a-clbB

    Plasmid

    #114155

    Purpose

    Expresses full-length ClbB

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbB
    

    Tags

    6xHis-thrombin-T7 tag

    Expression

    Bacterial

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TAL3126

    Plasmid

    #41300

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-nphs2-Left (nphs2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pETx-SR6v15.d

    Plasmid

    #176213

    Purpose

    Bacterial expression of the SR6v15.d targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6v15.d
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TAL2296

    Plasmid

    #36729

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BAX-TALEN_Left (Bax Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

18. TAL2297

    Plasmid

    #36730

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    BAX-TALEN_Right (BAX Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pPD3C

    Plasmid

    #86971

    Purpose

    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA3 fused to phosphite dehydrogenase and P450cam
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…

    Promoter

    T7 promoter

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRSET-GST-05230

    Plasmid

    #67874

    Purpose

    E.coli expression vector derived from pRSET encoding shy1 (um05230) with a N-terminal GST-fusion.

    Depositor

    Armin Djamei

    Article

    Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

    Insert

    shy1
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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