We narrowed to 5,770 results for: cki

1. pMK1253

   Plasmid

   #133058

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mClover2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNLS-2xBFP(4C)

   Plasmid

   #176151

   Purpose

   Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   NLS-2xBFP(4C)
   

   Use

   Tags

   6xHis tag and SV40 nuclear localization sequence …

   Expression

   Bacterial

   Mutation

   Compared with EGFP, the two BFP domains have the …

   Promoter

   Ptrc

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB44_pmc.DonorR5.TS

   Plasmid

   #199223

   Purpose

   Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJW1347

   Plasmid

   #154310

   Purpose

   Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa Linker for CT slot
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Myc-RILPL1

   Plasmid

   #201470

   Purpose

   For overexpression in cultured cells to visualize RILPL1 protein interactions.

   Depositor

   Suzanne Pfeffer

   Article

   Pal et al Sci Adv. 2023 Dec 15;9(50):eadj1205. doi

   Insert

   RILPL1 (RILPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPRv1 sgTollip

   Plasmid

   #196546

   Purpose

   Lentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mApple-M1 Spastin

   Plasmid

   #134461

   Purpose

   M1 Spastin expression

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

   Insert

   M1 Spastin (SPAST Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Met to Ala change at amino acid 87

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pM9-BetaGal-8C

   Plasmid

   #172489

   Purpose

   Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

   Depositor

   Siegfried Musser

   Article

   Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

   Insert

   M9-BetaGal-8C
   

   Use

   Tags

   6xHis and M9 nuclear localization sequence (from …

   Expression

   Bacterial

   Mutation

   BetaGal has two cysteine mutations (C77A, C1022S)…

   Promoter

   T7

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lenti-GBA-N370S-nick sgRNA

   Plasmid

   #214096

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   KRAS G13A nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTSK561

    Plasmid

    #190816

    Purpose

    For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    HaloTag
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge