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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-JNKKTRClover

Plasmid

#59139

Purpose

ORF encoding JNK KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen-WPRE

Plasmid

#193341

Purpose

AAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126218

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-155

Plasmid

#118537

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABECD

Plasmid

#66536

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Mutant T7 Promoter - G6

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-synp-F-H2B-jRGECO1a

Plasmid

#102995

Purpose

Expresses histone fused jRGECO1a under the synapsin promoter

Depositor

David Cox

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

H2B-jRGECO1a

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsZ

Plasmid

#89656

Purpose

Plasmid for titrating synthesis of FtsZ with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

GTP-binding tubulin-like cell division protein

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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Sf-U7snRNA-ESE-AS-NsiI

Plasmid

#190694

Purpose

Basic vector used for the expression of U7-based antisense oligonucleotides

Depositor

Pim Pijnappel

Article

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U7 promoter

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Use

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
SLF045: AAV-hSyn-eTsChR-GFP

Plasmid

#112280

Purpose

AAV based broad expression of eTsChR in neurons

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

eTsChR

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 K232R

Plasmid

#80878

Purpose

mammalian expression of ALK5 K232R

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K232R (kinase dead)

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hAsCpf1(TYCV) (pY210)

Plasmid

#89351

Purpose

Expresses humanized AsCpf1 variant that recognizes TYCV PAMs

Depositor

Feng Zhang

Article

Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

Insert

Cpf1, RR variant

Use

CRISPR

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

S542R/K607R

Promoter

CMV

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Use

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-Electra1

Plasmid

#179478

Purpose

Nuclear labeling with blue fluorescent protein Electra1 fused to H2B

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

H2B-Electra1

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24D

Plasmid

#66614

Purpose

YFP fusion of Sec24D

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24D (SEC24D Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag Jnk2a1

Plasmid

#13754

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a1 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only