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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 6

Plasmid

#236623

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1831-2663

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 2

Plasmid

#236619

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-1006

Available since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-puro-3xFLAG-ANKRD11-3xHA fragment 1

Plasmid

#236618

Purpose

Expresses human ANKRD11 fragment in mammalian cells

Depositor

Tadashi Nakagawa

Publication

Sashiyama et al iScience. 2025 May 19;28(6):112699. doi:

Insert

ANKRD11 (ANKRD11 Human)

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

aa 1-658

Available since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Puro-CMV-EPB41L4A-AS1 unspliced-WT

Plasmid

#242749

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Publication

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCLJ-P2F-Frt-PGK-Puro-Frt

Plasmid

#227501

Purpose

Inserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassette

Depositor

Joanna Wysocka

Publication

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Insert

Fgf5 promoter

Use

AAV and Mouse Targeting

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro GFP-REX1-IRES-mCherry

Plasmid

#232239

Purpose

Mammalian expression of REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Publication

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Publication

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #1-gRNA-Cas9-Puro

Plasmid

#186892

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse STING #2-gRNA-Cas9-Puro

Plasmid

#186893

Purpose

gRNA targeting mouse STING (with Cas9 insert)

Depositor

Jonathan Kagan

Publication

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Sting1 Mouse)

Use

Lentiviral

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4

Plasmid

#216823

Purpose

Expresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Publication

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A4

Expression

Mammalian

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8

Plasmid

#216824

Purpose

Expresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Publication

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8

Expression

Mammalian

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Publication

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Expression

Mammalian

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4

Plasmid

#216826

Purpose

Expresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Publication

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-4

Expression

Mammalian

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC

Plasmid

#179841

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc expression in human cells

Depositor

Xiaoping Bao

Publication

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-iChloC

Plasmid

#179842

Purpose

donor plasmid for cardiac-specific CCND2-T2A-iChloc expression in human cells

Depositor

Xiaoping Bao

Publication

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

improved chloride-conducting ChR (iChloC)

Expression

Mammalian

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-luciferase

Plasmid

#179844

Purpose

donor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cells

Depositor

Xiaoping Bao

Publication

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Tags

luciferase

Expression

Mammalian

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TetOne-Puro-p62 K7A/D69A

Plasmid

#204549

Purpose

Expresses p62 K7A/D69A in a doxycycline-dependent manner in mammalian cells

Depositor

Masaaki Komatsu

Publication

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K7A/D69A

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-P2A-puro

Plasmid

#192131

Purpose

Expresses Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Publication

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9

Use

AAV

Expression

Mammalian

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Hsp1-Hsp2Cas9-Y-P2A-puro

Plasmid

#192132

Purpose

Expresses highly specific Hsp1-Hsp2Cas9, cloning backbone for sgRNA and puromycin resistance gene

Depositor

Yongming Wang

Publication

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-Hsp2Cas9-Y

Use

AAV

Expression

Mammalian

Mutation

Hsp1-Hsp2Cas9 (Y446A)

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Ccu-sgRNA-SV40-puro

Plasmid

#192130

Purpose

Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Publication

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Ccu-SgRNA scaffold

Expression

Mammalian

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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