We narrowed to 12,952 results for: sequence

1. MTK2_026

   Plasmid

   #123718

   Purpose

   Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pSFFVc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Crippled Kozac sequence

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 8xCTS1_BpiI-cloning-site_SV40polyA

   Plasmid

   #124547

   Purpose

   Destination cloning vector for tRNA-flanked sgRNA. A BpiI placeholder is present between a minimal ADE promoter and SV40 polyA sequence.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

   Plasmid

   #49228

   Purpose

   Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

   Depositor

   Clifford Wang

   Article

   Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

   Inserts

   mEGFP
   mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SfuI site to create C-terminal fusions

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-short

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH375-SUP45-P174Q

   Plasmid

   #29383

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   P174Q mutant gene including genomic sequence 1432…

   Promoter

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pTH734-CEN-minHIS3

   Plasmid

   #38227

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Every codon of the coding sequence has been repla…

   Promoter

   TDH3

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH737-CEN-maxHIS3

   Plasmid

   #38229

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   HIS3 (HIS3 Budding Yeast, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Every codon of the coding sequence has been repla…

   Promoter

   TDH3 (=GPD)

   Available Since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-Flex-rev-GFPFANACfullWT

   Plasmid

   #126551

   Purpose

   AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

   Depositor

   Rory McQuiston

   Article

   Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

   Insert

   eGFP N-terminal fused to FMRFamide gate sodium channel
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK205

   Plasmid

   #110587

   Purpose

   BTK Type 3 coding sequence for golden gate assembly

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP optim-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pDONR P2R-P3-EYFP

   Plasmid

   #48351

   Purpose

   Contributes the coding sequence for EYFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Anna Planas, Tomas Santalucia

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EYFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Contains a stop codon.

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. peT3a-AqAdk_MVGDH

    Plasmid

    #18092

    Depositor

    Dorothee Kern

    Article

    Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

    Insert

    adenylate kinase (kad A. aeolicus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Tyr 52 changed to Cys, Val 145 changed to Cys, …

    Promoter

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

11. MTK4_004

    Plasmid

    #123813

    Purpose

    Encodes spacer sequence as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Spacer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2K-CAGGS-U6-sgRNA-M9-IRES-CFP

    Plasmid

    #114731

    Purpose

    sgRNA targeting a sequence upstream of the initiator ATG of the cellular Myc gene

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    sgRNA M9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(+)MBII-85-3'ss mut

    Plasmid

    #67644

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3' splice site muattion

    Promoter

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmEos2-Linker_PCNA-MutC

    Plasmid

    #98272

    Purpose

    for low level expression of mEos2-PCNA in mammalian cells

    Depositor

    Mike Heilemann

    Article

    Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

    Promoter

    CMV

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRPB1-CTD8

    Plasmid

    #112820

    Purpose

    pSMF2 with 8 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRPB1-CTD10

    Plasmid

    #112821

    Purpose

    pSMF2 with 10 CTD repeats

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CTD repeats have identical sequence, deleted repe…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCK025

    Plasmid

    #105415

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    Hormone-binding domain and regulatory sequences (estrogen receptor)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI/ BpiI restriction sites removed

    Promoter

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PFA0445w-bio-His

    Plasmid

    #50811

    Purpose

    Expresses enzymatically monobiotinylated full length PFA0445w

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PFA0445w
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PF10_0166-bio-His

    Plasmid

    #50801

    Purpose

    Expresses enzymatically monobiotinylated full length PF10_0166

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PF10_0166
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pWPT-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49232

    Purpose

    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-short

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRS424-TUB2 - human TUBB6 Cterminal tail

    Plasmid

    #60403

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pCru5-GGG/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49221

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCru5-UCU/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49223

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49219

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. mE-mCherry2A

    Plasmid

    #236248

    Purpose

    Middle entry fluorescent mCherry reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

    Depositor

    James Amatruda, Genevieve Kendall

    Article

    Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

    Insert

    mCherry2A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added a viral2A sequence to mCherry with no stop …

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACUH 5-HT2BR-7xGFP11-HA

    Plasmid

    #221840

    Purpose

    Plasmid for generating 10xUAS-5-HT2BR-7xGFP11-HA transgenic flies with attP/attB integration. It carries the attB sequence.

    Depositor

    Quentin Gaudry

    Article

    Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

    Insert

    5-HT2BR (CG42796 Fly)
    

    Use

    Tags

    7xGFP11-HA

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available Since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. M8

    Plasmid

    #180253

    Purpose

    Vaccine vector encoding 8 neonatigens specific for the MC38 colon cancer mouse model

    Depositor

    Fabio Palombo

    Article

    Salvatori et al NPJ Vaccines. 2022 Feb 2;7(1):15. doi: 1

    Insert

    Synthetic gene encoding for 8 neonatigens specific for MC38 mouse cancer cells
    

    Use

    Vaccine vector

    Tags

    Expression

    Mammalian

    Mutation

    the polyepitope vaccine encodes for mutated seque…

    Promoter

    CMV

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUBC(k)-AT5G05380-mKOk

    Plasmid

    #224849

    Purpose

    Plasmid for expression of AT5G05380.1 coding sequence tagged with mKOk in plants

    Depositor

    Rebecca Roston

    Article

    LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

    Insert

    AT5G05380.1 (PRA1.B3 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUBC(k)-AT5G07110-mKOk

    Plasmid

    #224850

    Purpose

    Plasmid for expression of AT5G07110.1 coding sequence tagged with mKOk in plants

    Depositor

    Rebecca Roston

    Article

    LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

    Insert

    AT5G07110.1 (PRA1.B6 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge