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We narrowed to 18,759 results for: nls

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Showing: 10321 - 10360 of 18759 results

  8. EFS-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222500
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222500-plasmid-map-sequence-id-441137

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  9. EFS-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222501
    Purpose
    Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Insert
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    EFS
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222501-plasmid-map-sequence-id-441220

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  10. TRE3GV-SpdCas9-Dnmt3A/3L-WT

    Plasmid
    #222502
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222502-plasmid-map-sequence-id-441221

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  11. pPB12A

    Plasmid
    #210035
    Purpose
    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas12a
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210035-plasmid-map-sequence-id-413996

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  14. pCJ1021

    Plasmid
    #228761
    Purpose
    Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.
    Depositor
    Bradley Yoder
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ift88 gRNAs (Ift88 Mouse)
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    228761-plasmid-map-sequence-id-455784

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  21. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

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  22. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

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  23. pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald

    Plasmid
    #225942
    Purpose
    Lentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis, Evan Reid
    Inserts
    RRBP1 (RRBP1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225942-plasmid-map-sequence-id-446793

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  24. pKSEe401R

    Plasmid
    #218532
    Purpose
    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.
    Depositor
    Kirill Demchenko
    Publication
    Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    218532-plasmid-map-sequence-id-458122

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  28. pCEFL-EGFP-TEADiv2

    Plasmid
    #228872
    Purpose
    Expression of optimized TEAD inhibitor (TEADiv2): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal
    Depositor
    Ramiro Iglesias-Bartolome
    Publication
    Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TEADiv2
    Tags
    GFP
    Expression
    Mammalian
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228872-plasmid-map-sequence-id-457051

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  30. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  31. pDual_dsCas9_Blast_hs_TP73

    Plasmid
    #214691
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human TP73
    Depositor
    Kevin Janes
    Insert
    dgRNA_TP73 (TP73 Human)
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214691-plasmid-map-sequence-id-428772

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  32. pDual_dsCas9_Venus

    Plasmid
    #214678
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Venus with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214678-plasmid-map-sequence-id-428813

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  34. eSpCas9-hGeminin

    Plasmid
    #199344
    Purpose
    modified version of the eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA plasmid (addgene #86613) where the C-terminus of the eSpCas9 enzyme was fused to amino acids 1 – 110 of human Geminin
    Depositor
    Iain Hagan
    Publication
    Morrison et al Open Biol. 2023 Apr;13(4):230021. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP1A1 G3 sgRNA+user-specified sgRNA+enhanced specificity Cas9 (1.1) (addgene 86613) with cas9 fused to hgem fragment 1-110) (GMNN S. pyogenes)
    Tags
    cas9 c-term fused to hgemenin 1-110 fragment
    Expression
    Mammalian
    Mutation
    cas9 c-term fused to hgemenin 1-110 fragment
    Promoter
    cbh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    199344-plasmid-map-sequence-id-402934

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  35. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  38. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  39. pAGK111

    Plasmid
    #228126
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228126-plasmid-map-sequence-id-455418

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  40. pDF850

    Plasmid
    #228713
    Purpose
    Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Publication
    Jiang et al Science 0, eadr6006
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    epPsaCas12f
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    I178A/K333V/K454P
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    228713-plasmid-map-sequence-id-454042

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Showing: 10321 - 10360 of 18759 results