We narrowed to 5,906 results for: pcDNA 3.1

1. pcDNA3.1(+)-R/G-ICUE

   Plasmid

   #181849

   Purpose

   ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   R/G-ICUE (RAPGEF3 Human)
   

   Use

   Tags

   mRuby2 and sfGFP

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-nCFP

   Plasmid

   #185360

   Purpose

   For mammalian expression of N-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-cCFP

   Plasmid

   #185361

   Purpose

   For mammalian expression of C-term CFP for split-CFP

   Depositor

   Ayaz Najafov

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-NKD2S31A

   Plasmid

   #174331

   Purpose

   encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.

   Depositor

   Sonal Srikanth

   Article

   Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

   Insert

   Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
   

   Use

   Tags

   MycHis

   Expression

   Mammalian

   Mutation

   changed Serine 31 to Alanine

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 4LNDD-pCDNA3.1(+)

   Plasmid

   #172227

   Purpose

   Expresses human ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) in mammalian cells

   Depositor

   Joshua Rosenthal

   Article

   Montiel-Gonzalez et al Nucleic Acids Res. 2016 Dec 1;44(21):e15

   Insert

   Lambda N peptide - human ADAR2 Deaminase Domain
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-rsAKARev(T/A)

   Plasmid

   #166232

   Purpose

   Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

   Depositor

   Peter Dedecker

   Article

   Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

   Insert

   Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Threonine 506 in rsAKARev to Alanine

   Promoter

   CMV

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-V5-SPR

   Plasmid

   #127236

   Purpose

   Eukaryotic expression of SPR

   Depositor

   Kai Johnsson

   Article

   Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

   Insert

   SPR (SPR Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_SNORD2 probe

   Plasmid

   #73070

   Purpose

   antisense probe for detection of human SNORD2 (C/D box snoRNA snR39B)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD2 (SNORD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1_SNORD60 probe

   Plasmid

   #73071

   Purpose

   antisense probe for detection of human SNORD60 (C/D box snoRNA U60)

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

   Insert

   SNORD60 (SNORD60 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   t7

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1+-NONHSAT073641

    Plasmid

    #92432

    Purpose

    mammalian expression of lncRNA NONHSAT073641

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    NONHSAT073641
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. miR373 in pcDNA3.1

    Plasmid

    #78127

    Purpose

    Mammalian expression of miR373

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    miR373 (MIR373 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. miR766 in pcDNA3.1

    Plasmid

    #78124

    Purpose

    Mammalian expression of miR766

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    miR766 (MIR766 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)mGAT1CFP45

    Plasmid

    #41676

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)mGAT1YFP45

    Plasmid

    #41675

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” YFP A206K mutation; 45 C-terminal-…

    Promoter

    CMV

    Available since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Precission-NEDD4L_FL_pCDNA3.1(+)

    Plasmid

    #187730

    Purpose

    Express NEDD4L FL in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Peredox-mCherry

    Plasmid

    #32383

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-2xFLAG-SREBP-1c

    Plasmid

    #26802

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1c (SREBF1 Human)
    

    Use

    Tags

    2XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-2xFLAG-SREBP-1a

    Plasmid

    #26801

    Depositor

    Timothy Osborne

    Article

    Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

    Insert

    SREBP-1a (SREBF1 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RGS-6xHis-pcDNA3.1-

    Plasmid

    #52534

    Purpose

    enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Type

    Empty backbone

    Use

    Tags

    RGS-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+)-RIα-EGFP

    Plasmid

    #181840

    Purpose

    GFP-tagged PKA RIα subunit for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-EGFP (PRKAR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge