We narrowed to 1,806 results for: pDest
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Plasmid#186377PurposeDestination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDEST27-GST-hSFRP1(aa181-314)
Plasmid#189548PurposeProtein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
TagsGSTExpressionMammalianMutationcontains aa181-314Available SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST27-GST-hSFRP1(aa53-169)
Plasmid#189545PurposeProtein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.DepositorInserttruncated secreted frizzled related protein 1 (SFRP1 Human)
TagsGSTExpressionMammalianMutationcontains aa53-169Available SinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA
Plasmid#186411PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mNeonGreen-stop
Plasmid#186392PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mEos4b-stop
Plasmid#186390PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mClover3-stop
Plasmid#186389PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mClover3 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Rfa-mCherry-stop
Plasmid#186388PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mCherry into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mVenus-RfA
Plasmid#186384PurposeDestination vector for the expression of a fluorescent fusion of N-ter mVenus and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mScarlet-I-RfA
Plasmid#186383PurposeDestination vector for the expression of a fluorescent fusion of N-ter mScarlet-I and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mNeonGreen-RfA
Plasmid#186382PurposeDestination vector for the expression of a fluorescent fusion of N-ter mNeonGreen and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mEos4b-RfA
Plasmid#186380PurposeDestination vector for the expression of a fluorescent fusion of N-ter mEos4b and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mClover3-RfA
Plasmid#186379PurposeDestination vector for the expression of a fluorescent fusion of N-ter mClover3 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-mCherry-RfA
Plasmid#186378PurposeDestination vector for the expression of a fluorescent fusion of N-ter mCherry and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA
Plasmid#186410PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorAvailable SinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM262 - pDESTR4-R3 mosTI hygroR target
Plasmid#159840PurposeSingle copy or array insertion by MosTI using split hygroR selectionDepositorInsertpDESTR4-R3 mosTI hygroR target
ExpressionWormMutationNot applicableAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-M135R-EGFP
Plasmid#164944PurposeTol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deleted; M135RPromoteractinb (actb2) promoterAvailable SinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP
Plasmid#164945PurposeTol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFPDepositorInsertKCNJ13 (KCNJ13 Human)
UseTol2 transposon destination vectorTagsIRES-EGFPMutationStop codon deletedPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-Q153H-EGFP
Plasmid#164943PurposeTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154IDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deleted; Q153H; M154IPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-T131A-EGFP
Plasmid#164942PurposeTol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133RDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deleted; T131A; G133RPromoteractinb (actb2) promoterAvailable SinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj1b-IRES-EGFP
Plasmid#164946PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFPDepositorInsertkcnj1b (kcnj1b Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Hemmar-DSCP-ftz-FLP(D)
Plasmid#119894PurposeFLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intronDepositorInsertsUseDrosophila expressionMutationa mutated form of FLP, which at position 5 contai…Available SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only