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pHES839

Plasmid

#87941

Purpose

Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

ADH1 (crippled)

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-B'-l2-B'

Plasmid

#209119

Purpose

Bacterial expression of CC-GEMS ligand SUMO-B'-l2-B'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-B'-l2-B'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-G'-l2-G'

Plasmid

#209120

Purpose

Bacterial expression of CC-GEMS ligand SUMO-G'-l2-G'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-G'-l2-G'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-pCMV-PfV-GS-Sapphire

Plasmid

#116933

Purpose

Mammalian vector for expression of the 40nm GEM

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-AqLumSynth –Sapphire

Plasmid

#116931

Purpose

Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

lumazine synthase

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-PfV-GS-Sapphire

Plasmid

#116932

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS305-Leu2-PINO4-PfV-GS-Sapphire

Plasmid

#116930

Purpose

Yeast integrative vector for the exression of the 40nm GEM under the INO4 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

INO4

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-PfV-Sapphire-IRES-DSRed

Plasmid

#116934

Purpose

Mammalian lenti vector for expression of the 40nm GEM and DSRed

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

Pfv

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G'-IRES-EmGFP

Plasmid

#209127

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l4-G-IRES-EmGFP

Plasmid

#209126

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l4-G and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l4-G-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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BBa_J72214-BBa_J72090

Plasmid

#40782

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

VioABCDE

Use

Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72100

Plasmid

#40781

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

lacZ

Use

Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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BBa_J72114-BBa_J72104

Plasmid

#40783

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

RhaRS + pRhaB + LuxCDABE, VioABCE, LacZ

Use

Luciferase and Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire

Plasmid

#203653

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pINO4-SV40NLS-PfV-GS-Sapphire

Plasmid

#203655

Purpose

Yeast integrative vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Tags

Sapphire

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC-SV40NLS-Pfv-Sapphire-PURO

Plasmid

#203654

Purpose

Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Lentiviral

Tags

Sapphire

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry

Plasmid

#203656

Purpose

Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Tags

yEmCherry

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pHIS3-SV40NLS-PfV-GS-GFPMUT3

Plasmid

#203657

Purpose

Yeast integrative vector for the expression of the GFPMUT3-tagged nuclear localizing 40nm nucGEMs under the HIS3 promoter.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

Tags

GFPMUT3

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pScaf

Plasmid

#111401

Purpose

Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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