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pvPE-V3-Puro

Plasmid

#240289

Purpose

Encodes third generation porcine endogenous retrovirus-derived prime editing (pvPE) system with puromycin resistance gene in to mammalian cells for gene editing.

Depositor

Lusheng Huang

Article

Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779

Insert

pvPE-V3-Puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-RasS17N

Plasmid

#214820

Purpose

Encoding dominant negative HRas

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative HRas

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rac1T17N

Plasmid

#214821

Purpose

Encoding dominant negative Rac1

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rac1

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-Rab5S34N

Plasmid

#214822

Purpose

Encoding dominant negative Rab5A

Depositor

Michiyuki Matsuda

Article

Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

Insert

Dominant negative Rab5

Expression

Mammalian

Promoter

CAG promoter

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24C #2

Plasmid

#231564

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#2) (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24C #2 (SEC24C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24C #1

Plasmid

#231563

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24C (#1) (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24C #1 (SEC24C Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgSEC24D

Plasmid

#231565

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human SEC24D (Puromycin selection marker)

Depositor

Rizwan Haq

Article

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgSEC24D (SEC24D Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_Myc-Sox9WT_PGKpuroR

Plasmid

#203962

Purpose

Dox inducible WT SOX9 with myc tag

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9 (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

Plasmid

#203963

Purpose

Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noTA (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the TA domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

Plasmid

#203964

Purpose

Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noHMG (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the HMG domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_FLAG-tNGFR_PuroR

Plasmid

#212651

Purpose

piggyBac vector to express FLAG-tNGFR with puromycin resistance

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

tNGFR

Use

Piggybac

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

Plasmid

#224388

Purpose

Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 7 domain deleted (aa 286-323)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

Plasmid

#224387

Purpose

Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 6 domain deleted (aa 242-282)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

Plasmid

#224385

Purpose

Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Expression

Bacterial and Mammalian

Mutation

WD 4 domain deleted (aa 153-195)

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5delta2CARD

Plasmid

#174225

Purpose

Expresses human MDA5 that lacks 2 CARD domains

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deletion of 2CARD

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1loop

Plasmid

#215032

Purpose

Mammalian expression of human TWIST1 (with NEUROD1 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa134-141, IIPTLPSD) replaced w…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROG2loop

Plasmid

#215033

Purpose

Mammalian expression of human TWIST1 (with NEUROG2 loop)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop (aa137-141, TLPSD) replaced with…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_V5-TWIST1_NEUROD1ins

Plasmid

#215036

Purpose

Mammalian expression of human TWIST1 (with insertion from NEUROD1)

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Tags

V5

Expression

Mammalian

Mutation

TWIST1 bHLH loop with insertion from NEUROD1 bHLH…

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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