We narrowed to 9,397 results for: bli

1. pYES2/NTA/Leu-LegA7ΔAnk290

   Plasmid

   #216520

   Purpose

   Expression of LegA7 ΔAnk290

   Depositor

   Ralph Isberg

   Insert

   LegA7 ΔAnk290
   

   Tags

   HIS-tag

   Expression

   Bacterial and Yeast

   Mutation

   LegA7 missing aa 290-454

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYES2/NTA/Leu-LegA7ΔNAnk264

   Plasmid

   #216521

   Purpose

   Expression of LegA7 ΔNAnk264

   Depositor

   Ralph Isberg

   Insert

   LegA7 ΔNAnk264
   

   Tags

   HIS-tag

   Expression

   Bacterial and Yeast

   Mutation

   LegA7 missing aa 264-361

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2/NTA/Leu-LegA7ΔNAnk290

   Plasmid

   #216522

   Purpose

   Expression of LegA7 ΔNAnk290

   Depositor

   Ralph Isberg

   Insert

   LegA7 ΔNAnk290
   

   Tags

   HIS-tag

   Expression

   Bacterial and Yeast

   Mutation

   LegA7 missing aa 290-361

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-RPL3L-TwStrep

   Plasmid

   #208649

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human RPL3L (ENST00000268661), contain SNP rs753338989, rs746044959, rs752134949

   Depositor

   Quanfu Ma

   Insert

   human RPL3L (ENST00000268661) (RPL3L Human)
   

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   contain rs753338989, rs746044959, rs752134949, pr…

   Promoter

   T7

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-K-red-SPOTIT(R271F)

   Plasmid

   #191494

   Purpose

   Red fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   K-red-SPOTIT(R271F)
   

   Use

   AAV

   Mutation

   R271F in OR

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET50_G495R MDA5

   Plasmid

   #216803

   Purpose

   Expresses a single point mutant of melanoma differentiation-associated protein 5

   Depositor

   Sun Hur

   Insert

   melanoma differentiation-associated protein 5 (IFIH1 Human)
   

   Expression

   Bacterial

   Mutation

   G495R

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIPZ-NUDT17-C-HA-Puro

   Plasmid

   #195182

   Purpose

   Lentiviral vector for DOX-inducible NUDT17 gene expression with C-terminal HA tag. Gateway expression system. Selection by puromycin.

   Depositor

   Stephanie Roessler

   Insert

   NUDT17 (NUDT17 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV promoter and minimal CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7CFE1_polio_3ABC_C147R

   Plasmid

   #212985

   Purpose

   Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   cysteine 147 in 3C changed to arginine

   Promoter

   T7

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7CFE1_EVA71_VP12A_C110A

   Plasmid

   #212976

   Purpose

   Encodes EV-A71 4643 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Enterovirus A71 4643 VP12A C110A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Mutation

   changed cysteine 110 from 2A to alanine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7CFE1_polio_VP12A_C109R

    Plasmid

    #212987

    Purpose

    Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus Type I Mahoney VP12A C109R
    

    Use

    Mammalian cell free protein expression

    Tags

    HA

    Mutation

    Cysteine 109 from 2A mutated to arginine

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCHA_SBV_NSs

    Plasmid

    #208938

    Purpose

    Expresses C-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cells

    Depositor

    Gerald Barry

    Insert

    NSs
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Deleted 47 bp at 5' end and 510 bp from 3&#x…

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGW-Fray

    Plasmid

    #203979

    Purpose

    GFP-Fray under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Frayed (fray Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAMW-WnkAAxV

    Plasmid

    #203980

    Purpose

    Myc6 Wnk under actin promoter for cell culture

    Depositor

    Andreas Jenny

    Insert

    Wnk (Wnk Fly)
    

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    RFxV mutated to AAxV

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-Cas9-Exo-Blast-BFP

    Plasmid

    #196721

    Purpose

    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-Exo1-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGB-06-07-Vps34

    Plasmid

    #187989

    Purpose

    Plasmid for the protein expression of Vps34 in a bacterial and insect expression system. Internal_Reference: SMC1323

    Depositor

    Sascha Martens

    Insert

    Vps34 (PIK3C3 Human)
    

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaMKII-Aion-ts-citrine-ER

    Plasmid

    #192576

    Purpose

    AAV-vector for optogenetic long-term silencing of excitatory neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    ts-Citrine-ER

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mDlx-Aion-citrine

    Plasmid

    #192572

    Purpose

    AAV-vector for optogenetic long-term silencing of GABAergic neurons

    Depositor

    Simon Wiegert

    Insert

    Aion
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, C128A,D156…

    Promoter

    minimal Dlx

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CaMKII-somBiPOLES-ChroME2s-mCerulean

    Plasmid

    #192582

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. CaMKII-somBiPOLES-ChroME2f-mCerulean

    Plasmid

    #192581

    Purpose

    AAV-vector for bidirectional optogenetic manipulation of excitatory neurons with blue shifted excitation spectrum

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    AAV

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Promoter

    CaMKIIa(0.4)

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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