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PB-TRE3G-TP53-CCRB

Plasmid

#236773

Purpose

A piggybac-based cloning vector containing TRE3G promoter-driven full-length TP53 and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.

Depositor

Kazutoshi Takahashi

Article

Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

Insert

TP53 (TP53 Human)

Use

Piggybac

Expression

Mammalian

Promoter

TRE3G promoter and TRE3G promoter, CAG promoter

Available Since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-TAZ-CAMTA1

Plasmid

#235679

Purpose

Express TAZ-CAMTA1 fusion gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

Insert

TAZ-CAMTA1 (WWTR1, CAMTA1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-FLAG-HAND1-T2A-mTagBFP2

Plasmid

#223192

Purpose

Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

Depositor

Matthew Birket

Article

Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

Insert

HAND1 (HAND1 Human)

Use

Promoterless entry vector for gateway cloning

Tags

FLAG and T2A-mTagBFP2

Expression

Bacterial

Promoter

None

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRP

Plasmid

#223728

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

GABARAP (GABARAP Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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CA245 CMVTO_ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry

Plasmid

#231206

Purpose

Single transcript cageIL-10 (mRens) + cleaveIL-6 (mRens) + ER-renin (H2M)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

ERret-H2M-Renin_P2A_mRens-hIL6-rens.1_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-K82H-CR

Plasmid

#229425

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-K82H (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Kinase-dead mutant K82H; silent mutation CRISPR r…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-MARK2-GK-CR

Plasmid

#229423

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MARK2-GK-CR (MARK2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Gatekeeper mutation M129G; silent mutation CRISPR…

Promoter

EFS

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Expression

Mammalian

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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PD2529 human beta1 ecto

Plasmid

#221398

Purpose

Mammalian expression of human integrin beta1 ectodomain

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 ectodomain (ITGB1 Human)

Tags

AVI tag, HRV3C cleavage site, basic coil, HA tag,…

Expression

Mammalian

Mutation

codon optimized for human mature _1 residues Q1 t…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(CD45TM)

Plasmid

#223629

Purpose

Lentiviral expression of human CD28 (CD45TM) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD28(CD45TM)

Plasmid

#223624

Purpose

Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Mutation

CD28 transmembrane domain replaced by CD45 transm…

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86(F56A)

Plasmid

#223609

Purpose

Lentiviral expression of human CD86 (F56A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

F56A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD80(Y65A)

Plasmid

#223608

Purpose

Lentiviral expression of human CD80 (Y65A) with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Mutation

Y65A

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-CD28(SGGG)

Plasmid

#223607

Purpose

Lentiviral expression of human CD28 (SGGG) with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Mutation

MYPPPY mutated to SGGG

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

Plasmid

#223582

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80 (I92R)-His10

Plasmid

#223580

Purpose

Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

I92R

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80-His10

Plasmid

#223579

Purpose

Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroSCRAMBLED

Plasmid

#215594

Purpose

For overexpression of Gal4-DBD EGR1 AroSCRAMBLED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroSCRAMBLED (EGR1 Human)

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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