We narrowed to 146,921 results for: ins;

1. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a Gx-mNG-NL

   Plasmid

   #169106

   Purpose

   Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - mNeonGreen - NanoLuc
   

   Use

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRVL-3

   Plasmid

   #104581

   Purpose

   Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L234A/L235E/G237A and E318A/K320A/K322A

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2Bc-T MBP_BoMoC(ed)_W403A_F753A_6xH

   Plasmid

   #185713

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed W403 and F753 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3-myc3-ROC1

   Plasmid

   #20717

   Depositor

   Yue Xiong

   Article

   He et al Genes Dev. 2006 Nov 1. 20(21):2949-54.

   Insert

   RING finger protein 1 (ROC1) (RBX1 Human)
   

   Use

   Tags

   3xMyc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEM318 - sgRNA mosTI unc-119 single-copy

   Plasmid

   #159822

   Purpose

   Single copy or array insertion by MosTI using split unc-119 selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI unc-119 single-copy
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV500 A-FOS

   Plasmid

   #33353

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   FOS (Fos Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative of c-Jun; heterodimerizes and i…

   Promoter

   CMV

   Available Since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRVL-5

    Plasmid

    #104583

    Purpose

    Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

    Depositor

    Daniel Christ

    Article

    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

    Insert

    Human IgG1 CH1/CH2/CH3 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BPK3082

    Plasmid

    #78742

    Purpose

    Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    LbCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII_TOPO_hNEAT1

    Plasmid

    #61518

    Purpose

    human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

    Depositor

    Archa Fox

    Article

    Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

    Insert

    NEAT1 (NEAT1 Human)
    

    Use

    General cloning vector

    Tags

    Expression

    Mutation

    *see comment below

    Promoter

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEM319 - array co-injection fragment mosTI unc-119

    Plasmid

    #159823

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI unc-119
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 3xFlag IFIT1

    Plasmid

    #53554

    Purpose

    mammalian expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL7.0: tgRFPt-SSPB WT

    Plasmid

    #60415

    Purpose

    Mammalian Expression of tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    Y11K and A15E

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. f-CTS-EGFP

    Plasmid

    #86064

    Purpose

    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains ciliary targeting signal (3870-3887 amin…

    Promoter

    CMV

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BPK3079

    Plasmid

    #78741

    Purpose

    Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    AsCpf1 crRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GST-p25

    Plasmid

    #24896

    Depositor

    Harish Pant

    Article

    Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

    Insert

    p25 (CDK5R1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    N-terminal truncated version of p35, contains aa …

    Promoter

    Available Since

    May 17, 2010

    Availability

    Academic Institutions and Nonprofits only