We narrowed to 143,240 results for: ins

1. pSCM167-Venus-VF-hADAM10(aa220-654)

   Plasmid

   #189560

   Purpose

   Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

   Depositor

   Haian Fu

   Insert

   truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   VF

   Expression

   Mammalian

   Mutation

   contains aa 220-654

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV human IRS-1 CAAX

   Plasmid

   #11238

   Depositor

   Ronald Kahn

   Article

   Kriauciunas et al Mol Cell Biol. 2000 Sep . 20(18):6849-59

   Insert

   IRS-1 (IRS1 Human)
   

   Use

   Tags

   CAAX

   Expression

   Mammalian

   Mutation

   C-terminal prenylation motif makes IRS-1 localize…

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pJ23119-sgRNA

   Plasmid

   #113654

   Purpose

   sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

   Depositor

   Jeffrey Barrick

   Article

   Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

   Insert

   promoter J23119 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PKG I alpha dNPS

   Plasmid

   #16391

   Depositor

   Bernard Weinstein

   Article

   Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

   Insert

   PKG1 alpha (PRKG1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N-terminal pseudosubstrate domain deletion: delet…

   Promoter

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pFUGW-ZipACR-EYFP

   Plasmid

   #88844

   Purpose

   Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

   Depositor

   John Spudich

   Article

   Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

   Insert

   ZipACR-NotI-EYFP
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP2262

   Plasmid

   #70703

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A & H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNP1 eforRed toehold switch

   Plasmid

   #107353

   Purpose

   Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 1 + eforRed
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vh-HA-FKBP

   Plasmid

   #20220

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   FKBP (FKBP1A Human)
   

   Use

   Tags

   HA and Vh

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. IFI16-(192-393)-HinA (pcDNA3-FLAG)

   Plasmid

   #35062

   Depositor

   Cheryl Arrowsmith

   Article

   Liao et al Structure. 2011 Mar 9;19(3):418-29.

   Insert

   IFI16-(192-393)-HinA (IFI16 Human)
   

   Use

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   Contains HinA domain: aa 192-393

   Promoter

   CMV

   Available since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

10. IFI16-(515-710)-HinB (pcDNA3-FLAG)

    Plasmid

    #35063

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16-(515-710)-HinB (IFI16 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains HinB domain: aa 515-710

    Promoter

    CMV

    Available since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

11. 2XMyc-LRRK2-Kinase

    Plasmid

    #25071

    Depositor

    Mark Cookson

    Article

    J Biol Chem. 2008 Jun 13;283(24):16906-1

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    2XMyc

    Expression

    Mammalian

    Mutation

    Contains only the kinase domain aa 1863-2218

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. AICSDP-83:EZH2-mEGFP

    Plasmid

    #164499

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human EZH2

    Depositor

    Allen Institute for Cell Science

    Insert

    EZH2 Homology Arms with mEGFP-linker (EZH2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pFUGW-RapACR-EYFP

    Plasmid

    #111075

    Purpose

    Fast anion channelrhodopsin (current half-decay time 3-10 ms depending on voltage and light intensity) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RapACR
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACE-GIRK4-S

    Plasmid

    #172425

    Purpose

    Expression of full-length, human GIRK4 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK4 (KCNJ5 Human)
    

    Use

    Tags

    Streptag II

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEAK-bKal7

    Plasmid

    #145800

    Purpose

    expresses Kalirin-7 with the b-front in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

    Insert

    bKalirin-7 (Kalrn Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EIF2a

    Available since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 sf:mid.11

    Plasmid

    #191921

    Purpose

    Encodes a GFP design, sf:mid.11

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.11
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69M Y145F T167I L220V V224I

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28 sf:mid.7

    Plasmid

    #191933

    Purpose

    Encodes a GFP design, sf:mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.7
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28 acid.6

    Plasmid

    #191912

    Purpose

    Encodes a GFP design, acid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.6
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72A Y145M T167V H181L

    Promoter

    T7

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28 sf:acid.3

    Plasmid

    #191926

    Purpose

    Encodes a GFP design, sf:acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:acid.3
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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