We narrowed to 6,013 results for: cki

1. CAG-Cremyc-2A-GFP

   Plasmid

   #116879

   Purpose

   Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

   Depositor

   Qiang Lu

   Article

   Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

   Insert

   Cremyc-2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-opto-hRIP3 dRHIM

   Plasmid

   #208788

   Purpose

   Inducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2

   Depositor

   Petr Broz

   Article

   Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   RIP3 (RIPK3 Human)
   

   Use

   Lentiviral

   Tags

   Cry2 mCherry

   Expression

   Mammalian

   Mutation

   RHIM domain is removed

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sp-sgRNA-mDNMT1_nick-lenti

   Plasmid

   #135959

   Purpose

   S. pyogenes nicking sgRNA for mouse DNMT1

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   mDNMT1_nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-DO-EGFP-WPRE-pA

   Plasmid

   #37085

   Purpose

   Expresses EGFP in cells lacking Cre (Cre-Off)

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   EGFP
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-RAPH1ΔTBS

   Plasmid

   #194859

   Purpose

   Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 2-92 deleted

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCPP5154

   Plasmid

   #128704

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   avrPto
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCPP5616

   Plasmid

   #128727

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   avrPtoB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_2FLAG-AKAP79ΔPKA

   Plasmid

   #183452

   Purpose

   Expression of human AKAP79 lacking the PKA binding site (Δ391-400)

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Use

   Tags

   2xFLAG

   Expression

   Mammalian

   Mutation

   aa391-400 deleted

   Promoter

   CMV

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-nuc-Nb80

   Plasmid

   #205519

   Purpose

   Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb80
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggybac-EF1-H2AZ-halo

    Plasmid

    #197907

    Purpose

    single-particle tracking for H2AZ

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    H2az2 (H2az2 Mouse)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH_Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65258

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP3416

    Plasmid

    #128720

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopT1-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPP5534

    Plasmid

    #128723

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopX1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-mCherry-Rheb(5A)

    Plasmid

    #192439

    Purpose

    Encodes mCherry-tagged Rheb lacking residues 38-42.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Rheb amino acids 38-42 deleted.

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DO-mCherry-WPRE-pA

    Plasmid

    #37119

    Purpose

    Expresses mCherry in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBMN-AS-HNF4α

    Plasmid

    #139305

    Purpose

    Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

    Depositor

    Claes Wadelius

    Article

    Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

    Insert

    shRNA and amiRNA against HNF4α
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3_2FLAG-AKAP79ΔCN

    Plasmid

    #183451

    Purpose

    Expression of human AKAP79 lacking the calcineurin binding site (Δ337-343)

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    aa337-343 deleted

    Promoter

    CMV

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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