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pCAG-FLAG-IRES-blasticidin-Sox2 S248A

Plasmid

#182047

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S248A

Promoter

CAG

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLAG-IRES-blasticidin-Sox2 T258A

Plasmid

#182048

Purpose

expression of Sox2 mutants

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

Insert

Sox2 (Sox2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

T258A

Promoter

CAG

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgMARK4

Plasmid

#138691

Purpose

Expresses a human MARK4-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgMARK4 human (MARK4 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-DICER-Prom Ebox double mut

Plasmid

#25854

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

DICER-Promoter Ebox double mut (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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MAC-CLEC4D

Plasmid

#172411

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member D (CD antigen CD368) (CLEC4D Human)

Tags

MAC-tag

Expression

Mammalian

Promoter

CMV promoter

Available Since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-DICER-Prom upstream Ebox Mut

Plasmid

#25852

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

DICER-Promoter upstream Ebox Mut (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37 SHPmut

Plasmid

#113503

Purpose

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

F215A/E218A/QKL220-222AAA

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-DICER-Prom downstream Ebox Mut

Plasmid

#25853

Depositor

David E. Fisher

Article

Levy et al Cell. 2010 Jun 11. 141(6):994-1005.

Insert

DICER-Prom downstream Ebox Mut (DICER1 gene ID 23405, Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pCDF1-GRM3 (S610L)

Plasmid

#31800

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed serine 610 to leucine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (E870K)

Plasmid

#31802

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 870 to lysine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGDF11 #1

Plasmid

#83083

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11, Gdf11 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (E767K)

Plasmid

#31801

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glutamic acid 767 to lysine

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-GRM3 (G561E)

Plasmid

#31799

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2011 Sep 25;43(11):1119-26. d

Insert

GRM3 (GRM3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed glycine 561 to glutamic acid

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 p37 deltaUBX

Plasmid

#113504

Purpose

Bacterial expression of GST-tagged p37 lacking the UBX domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 251-331 (stop codon behind AA250)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMiR-CIP2A(partial)

Plasmid

#53705

Purpose

Luciferase reporter assay for microRNA binding on CIP2A coding sequence

Depositor

Edward Chan

Article

Jung et al Mol Biol Cell. 2013 Jun;24(11):1638-48,

Insert

CIP2A (CIP2A Human)

Use

Luciferase

Available Since

Aug. 28, 2014

Availability

Academic Institutions and Nonprofits only
psiCHECK2-CCNA2 LONG 3'UTR

Plasmid

#29472

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

CCNA2 LONG 3'UTR (CCNA2 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Article

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSico-syn-GFP-TVA-oG-WPRE-bGHpA

Plasmid

#231009

Purpose

Lentiviral helper virus for G-deleted rabies tracing

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Inserts

Enhanced GFP
TVA (CD320 Chicken)
optimized G

Use

Lentiviral

Promoter

human synapsin I

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Lyn-SH2-AviTag

Plasmid

#214207

Purpose

Bacterial expression of SH2 domain of the Lyn kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Lyn A (LYN Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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