We narrowed to 18,662 results for: Mut

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pRS416Gal TDP43 WT YFP

Plasmid

#27447

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pCEP4-ZM197M-gp160

Plasmid

#123226

Purpose

Mammalian expression plasmid for Env from the ZM197M HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (ZM197M) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-AcGFP-progerin

Plasmid

#86947

Purpose

Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

Depositor

Alex Hewitt

Insert

AcGFP-progerin (LMNA Human)

Use

AAV

Tags

AcGFP

Expression

Mammalian

Mutation

progerin sequence is LMNA gene with nucleotide C1…

Promoter

CMV and T7

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE342

Plasmid

#153251

Purpose

M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-Q769gp160

Plasmid

#100928

Purpose

Mammalian expression plasmid for Env from the Q769.d22 HIV-1 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (Q769.d22) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE497

Plasmid

#153249

Purpose

M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-lox-GFP shRNA p19-2

Plasmid

#14091

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCAG-delta90GFP

Plasmid

#26645

Depositor

Anjen Chenn

Article

Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

Insert

delta90 beta-catenin (Ctnnb1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of first 90 aa of beta catenin

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BACK))

Plasmid

#169722

Purpose

Mammalian Expression of mouse SPOP Y353E

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y353E

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

Plasmid

#22131

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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μ2-HA-D176A

Plasmid

#32754

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Tags

HA

Expression

Mammalian

Mutation

D176A

Promoter

CMV

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
HA, EGFP Spastin M87 with exon 4

Plasmid

#167542

Purpose

For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

Depositor

Tarun Kapoor

Article

Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

Insert

Spastin M87 with exon 4 (SPAST Human)

Tags

EGFP and HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHDE-35S-Cas9-mCherry

Plasmid

#78931

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

Depositor

Yunde Zhao

Article

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sapphireCKAR(T/A)

Plasmid

#118469

Purpose

Negative-control mutant for sapphireCKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireCKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBEL2319

Plasmid

#195697

Purpose

Cryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

Foldon-MlaC

Expression

Bacterial

Mutation

6xHis-Foldon-(Gly-Ser-Linker)-MlaC

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

Plasmid

#116884

Purpose

FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RLR)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-NG-PE2-BSD

Plasmid

#176933

Purpose

Expresses PE2-NG variant in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Insert

PE2-NG variant

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pETCK3 TGP-E

Plasmid

#236255

Purpose

Expresses TGP-E, a thermostable fluorescent protein

Depositor

Natasha DeVore

Article

Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

Insert

Thermal Green Protein Q66E (TGP-E)

Tags

His tag

Expression

Bacterial

Promoter

lac operon

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hL1

Plasmid

#89411

Purpose

mammalian expression of hL1

Depositor

Fritz Rathjen

Article

De Angelis et al EMBO J. 1999 Sep 1. 18(17):4744-53.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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