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pME-LiCre (JDW 1119)

Plasmid

#242560

Purpose

Gateway middle entry clone containing a light inducible Cre Recombinase (LiCre).

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LiCre (AsLOV2-CreE340AD341A)

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-miR-MCS (JDW 1231)

Plasmid

#242562

Purpose

Gateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with artifial intron and MCS

Use

Gateway subcloning

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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MB 101A ttrSR(m13)-sfGFP_mCherry

Plasmid

#232474

Purpose

Optimized tetrathionate sensor with fluorescent reporter (GFP), constitutive mCherry

Depositor

Mikhail Shapiro

Article

Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:

Inserts

ttrS
ttrR
PttrB185-269
sfGFP
AxeTxe
mCherry

Expression

Bacterial

Promoter

Constitutive (TTGATAGCTAGCTCAGTCCTAGGTATTGTGCTAGC…

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-DreERT2 (JDW 196)

Plasmid

#242586

Purpose

The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

Plasmid

#242589

Purpose

A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh (actin nanobody)

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-deLACCO1-COBRA

Plasmid

#223344

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

deLACCO1

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-eLACCO2-COBRA

Plasmid

#223345

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-gfaABC1D-IgK-R-eLACCO2.1-COBRA

Plasmid

#223346

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACC02.1

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-IgK-R-eLACCO2-COBRA

Plasmid

#223348

Purpose

Biosensor for extracellular L-lactate

Depositor

Marie-Eve Paquet

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2

Use

AAV

Tags

GPI-COBL9 Anchor

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-zFUCCI (JDW 1464)

Plasmid

#242558

Purpose

Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mStayGold-WPRE (JDW 1384)

Plasmid

#242550

Purpose

Gateway middle entry clone containing H2B-mStayGold with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mStayGold (monomeric StayGold)

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME-mCerulean3-TUBB1A (JDW 1508)

Plasmid

#242555

Purpose

Gateway middle entry clone containing mCerulean3-TUBB1A; Cyan fluorescent reporter for visualizing tubulin in cells

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean3-TUBB1A

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 K26/27Q IRES Cherry

Plasmid

#228112

Purpose

Lentiviral expression of human INTS4 K26/27Q

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutated Lysine 26 and 27 to Glutamine

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP11-22-122-EGFP-Hygro

Plasmid

#238097

Purpose

Expression of structurally predicted WWE domain of PARP11 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP11 (PARP11 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 22-112 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-PARP12-298-462-Hygro

Plasmid

#238098

Purpose

Expression of structurally predicted WWE domain of PARP12 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP12 (PARP12 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 298-462 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-RNF146-45-182-EGFP-Hygro

Plasmid

#238091

Purpose

Expression of structurally predicted WWE domain of RNF146 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146 (RNF146 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 45-182 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX1-1-175-EGFP-Hygro

Plasmid

#238093

Purpose

Expression of structurally predicted WWE domain of DTX1 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX1 (DTX1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-175 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-DTX4-1-166-EGFP-Hygro

Plasmid

#238095

Purpose

Expression of structurally predicted WWE domain of DTX4 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX4 (DTX4 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-166 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-36XMYC array

Plasmid

#236380

Purpose

Expression of array of 36 crRNAs targeting MYC

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

36xMYC crRNA array (MYC Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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