We narrowed to 2,784 results for: aen

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B1

   Plasmid

   #165082

   Purpose

   gRNA 1 of pair B for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-2XFL-Src-K298M-Y419F-Y530F

   Plasmid

   #140321

   Purpose

   Expresses Flag-tagged human Src-K298M-Y419F-Y530F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   K298M-Y419F-Y530F

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM-U6-reRNA-hsHEKsite_1_3

   Plasmid

   #205638

   Purpose

   reRNA guide targeting HEKsite_1_3

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   reRNA-HEKsite_1_3 (EPHA3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOD2046-bwmDEG

   Plasmid

   #89367

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Pmyo-3

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSicoR p53

   Plasmid

   #12090

   Depositor

   Tyler Jacks

   Article

   Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

   Insert

   p53 shRNA (Trp53 Mouse)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   EGFP is expressed from this plasmid as a marker, …

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. hum alpha ENAC promoter a25-a23

   Plasmid

   #83427

   Purpose

   promoter reporter construct to test 5' flanking sequences

   Depositor

   Christie Thomas

   Article

   Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

   Insert

   human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mutation

   Promoter

   alpha ENAC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Stx5S-GFP

   Plasmid

   #209847

   Purpose

   Expresses Stx5S-GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Lacks amino acids 1-54, encodes short isoform of …

   Promoter

   CMV

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCC324

   Plasmid

   #110165

   Purpose

   miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

   Depositor

   Iva Greenwald

   Article

   de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

   Insert

   ERK-KTR-mClover-T2A-mCherry-H2B
   

   Use

   Minimos transposon

   Tags

   mCherry and mClover

   Expression

   Worm

   Mutation

   R61K change made in ERK-KTR CDS

   Promoter

   lin-31 promoter/enhancer

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOD2048-csnDEG

   Plasmid

   #89368

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Posm-6

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pGL4.15_pSOX17

    Plasmid

    #195496

    Purpose

    Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    Promoter region of SOX17 (SOX17 Human)
    

    Use

    Tags

    luc2-hPEST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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